NIST8375

Microbial Genomic DNA Standards for Sequencing Performance Assessment

(MG-001, MG-002, MG-003, MG-004)

• UNSPSC Code: 41116107
• NACRES: NA.24

Properties

• grade: certified reference material
• manufacturer/tradename: NIST^®
• technique(s): electrophoresis: suitable
• application(s): genomic analysis
• format: multi-component solution

Description

General description

This microbial genomic DNA standard is a reference material (RM) that consists of four vials. The genomic DNA is extracted from cell cultures. Each vial contains approximately 2 µg of microbial genomic DNA in TE buffer (10 mM TRIS, 1 mM EDTA, pH 8.0).

• MG-001 Salmonella Typhimurium LT2
• MG-002 Staphylococcus aureus
• MG-003 Pseudomonas aeruginosa
• MG-004 Clostridium sporogenes

NIST8375_Cert

NIST8375_SDS

Application

This reference material (RM) is suitable for validation, optimization, process evaluation, and performance assessment of microbial whole genome sequencing.

Features and Benefits

• Enables the assessment of the performance of high-throughput microbial DNA sequencing methods.
• It does not assess pre-analytical steps such as DNA extraction as the RM is extracted DNA.
• Challenges sequencing library preparation, sequencing machines, base calling algorithms, and subsequent bioinformatics analyses.
• This genomic DNA is extracted from cell cultures.
  

Other Notes

• Refer to the SDS for additional information on biomaterials, disposal, and transport.
• Details on expiration, maintenance, usage, storage, preparation, stability, homogeneity, and purity are available in the certificate.

Legal Information

NIST is a registered trademark of National Institute of Standards and Technology

Safety Information

• Storage Class Code: 10 - Combustible liquids