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H6378

Supelco

20α-Hydroxycholesterol

analytical standard

Synonym(s):

5-Cholestene-3β,20α-diol

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About This Item

Empirical Formula (Hill Notation):
C27H46O2
CAS Number:
516-72-3
Molecular Weight:
402.65
MDL number:
MFCD16661190
UNSPSC Code:
41116107
PubChem Substance ID:
329815230
NACRES:
NA.24

grade

analytical standard

Quality Level

200

Assay

≥98%

technique(s)

HPLC: suitable
gas chromatography (GC): suitable

application(s)

clinical testing

format

neat

SMILES string

CC(C)CCC[C@](C)(O)[C@H]1CC[C@H]2[C@@H]3CC=C4C[C@@H](O)CC[C@]4(C)[C@H]3CC[C@]12C

InChI

1S/C27H46O2/c1-18(2)7-6-14-27(5,29)24-11-10-22-21-9-8-19-17-20(28)12-15-25(19,3)23(21)13-16-26(22,24)4/h8,18,20-24,28-29H,6-7,9-17H2,1-5H3/t20-,21-,22-,23-,24-,25-,26-,27-/m0/s1

InChI key

MCKLJFJEQRYRQT-APGJSSKUSA-N

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Application

20α-Hydroxycholesterol has been used as a standard to determine the concentration of 20α-hydroxycholesterol, present in food samples using gas chromatography with an ion trap detector coupled with mass spectrometer (GC-IT-MS).
Refer to the product′s Certificate of Analysis for more information on a suitable instrument technique. Contact Technical Service for further support.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000276072
SLCD0481
SLBZ8817
SLBX0926
SLBV4688

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Cholesterol Sigma Grade, ≥99%

Sigma-Aldrich

C8667

Cholesterol

P B Hoyer et al.
Canadian journal of physiology and pharmacology, 63(3), 240-248 (1985-03-01)
Ovine luteal tissue contains two distinct steroidogenic cell types, small (8-20 microns) and large (greater than 20 microns), which differ based on morphological and biochemical criteria. Unstimulated small cells secrete low levels of progesterone, respond to LH or dibutyryl cAMP
J H Nielsen et al.
The Journal of dairy research, 62(1), 101-113 (1995-02-01)
A method for isolation, detection and quantification of cholesterol oxidation products based on solid phase extraction in combination with preparative HPLC and gas chromatography-mass spectrometry selected ion monitoring has been developed for dairy products. The isolation procedure had a high
T Y Wing et al.
Endocrinology, 117(5), 1779-1787 (1985-11-01)
In the present study, we explored the restoration effects of exogenous LH on Leydig cell ultrastructure and testicular steroidogenesis in rats that were deprived of endogenous LH via treatment with testosterone-17 beta-estradiol-filled Silastic implants for 10 days. Exogenous LH was
K Z Liu et al.
American heart journal, 123(2), 285-290 (1992-02-01)
Oxidized low-density lipoprotein (LDL) may play an important role in atherogenesis. The oxidative status of isolated LDL and very low-density lipoprotein (VLDL) were investigated in 23 patients with proven coronary disease and in 23 healthy asymptomatic control subjects. Oxidized cholesterol
M W Rooney et al.
Biochimica et biophysica acta, 820(1), 33-39 (1985-10-24)
Fourier transform infrared spectroscopy revealed that insertion of 20 alpha-hydroxycholesterol into human erythrocyte membranes (10% of total membrane sterol) immobilized the lipid acyl chains to a degree equivalent to enriching total membrane cholesterol by 50% (Rooney, M.W., Lange, Y. and
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