70191

Mueller Hinton Agar

suitable for microbiology, NutriSelect^® Plus

• Synonym(s): MH agar; Antibiotic sensitivity test media; disc diffusion test media
• UNSPSC Code: 41171607
• NACRES: NA.74

Properties

• sterility: non-sterile
• Quality Level: 200
• product line: BioChemika
• form: powder
• shelf life: limited shelf life, expiry date on the label
• composition: agar, 17.0 g/L ; beef infusion solids, 2.0 g/L ; casein hydrolysate, 17.5 g/L ; starch, 1.5 g/L
• manufacturer/tradename: NutriSelect^® Plus
• technique(s): microbe id | susceptibility testing: suitable; microbiological culture: suitable
• final pH: 7.3±0.2 (25 °C)
• application(s): clinical testing; food and beverages; microbiology
• storage temp.: 10-30°C
• suitability: nonselective for Neisseria spp.; nonselective for bacteria (General Media)

Description

General description

Mueller Hinton Agar is specially designed for antibiotic susceptibility testing using the disc diffusion method (Kirby Bauer method). It has been recommended by the CLSI as the ideal medium for antibiotic susceptibility testing for the following reasons:

• It shows good reproducibility from batch to batch
• It is low in certain inhibitors like trimethoprim, sulfonamide and tetracycline
• It supports the growth of both fastidious and non-fastidious bacterial pathogens
• The starch in the media absorbs toxins thus preventing them from interfering with the antibiotics
• It is a loose agar and therefore facilitates better diffusion of antibiotics

Mueller Hinton Agar has been used:

• In culturing Escherichia coli ATCC™ 25922 strain for disc diffusion studies
• In disk diffusion assay of fusidic acid against Staphylococcus aureus, Bacillus cereus, Pseudomonas aeruginosa and Escherichia coli
• In agar diffusion assay of drug-loaded nano-hydroxyapatite formulations against Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli

Application

Mueller Hinton Agar is a solid medium originally designed for the isolation of pathogenic Neisseria species, now widely used for antibiotic susceptibility testing (including sulfonamides) of aerobic and facultatively anaerobic bacteria isolated from clinical specimens.

Preparation Note

Suspend 38 g in 1 litre of distilled water, bring to the boil to dissolve the medium completely and sterilize by autoclaving at 121°C for 15 minutes.

Other Notes

Enhancement of cefotoxamine and other cephalosporins against Enterococcus faecalis by blood supplemented Mueller-Hinton agar

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves