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63183

Sigma-Aldrich

N-(3-Maleimidopropionyl)biocytin

≥75% (HPLC)

Synonym(s):

MPB, Nε-Biotinyl-Nα-(3-maleimidopropionyl)-L-lysine

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About This Item

Empirical Formula (Hill Notation):
C23H33N5O7S
CAS Number:
Molecular Weight:
523.60
MDL number:
UNSPSC Code:
12352200
PubChem Substance ID:
NACRES:
NA.25
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Quality Level

Assay

≥75% (HPLC)

SMILES string

OC(=O)[C@H](CCCCNC(=O)CCCC[C@@H]1SC[C@@H]2NC(=O)N[C@H]12)NC(=O)CCN3C(=O)C=CC3=O

InChI

1S/C23H33N5O7S/c29-17(7-2-1-6-16-21-15(13-36-16)26-23(35)27-21)24-11-4-3-5-14(22(33)34)25-18(30)10-12-28-19(31)8-9-20(28)32/h8-9,14-16,21H,1-7,10-13H2,(H,24,29)(H,25,30)(H,33,34)(H2,26,27,35)/t14-,15-,16-,21-/m0/s1

InChI key

KWNGAZCDAJSVLC-OSAWLIQMSA-N

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Application

N-(3-Maleimidopropionyl)biocytin (MPB) is a versatile biotinylating reagent and thiol-specific probe that can detect protein SH groups on dot blots in the fmole range. MPB can be used for protein blotting, enzyme immunoassay, protein immobilization and various cytochemical procedures.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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T Wada et al.
The Journal of biological chemistry, 274(24), 17353-17357 (1999-06-08)
Cysteine mutagenesis and surface labeling has been used to define more precisely the transmembrane spans of subunit a of the Escherichia coli ATP synthase. Regions of subunit a that are exposed to the periplasmic space have been identified by a
E A Bayer et al.
Analytical biochemistry, 161(2), 262-271 (1987-03-01)
Various strategies for the use of 3-(N-maleimido-propionyl) biocytin (MPB) as a general label for distinguishing between protein sulfhydryls and disulfides on blot transfers are presented. In the first approach, endogenous SH groups in proteins were labeled directly with MPB. For
E Roffman et al.
Biochemical and biophysical research communications, 136(1), 80-85 (1986-04-14)
Amino groups, sulfhydryl groups or oxidation-induced aldehydes on erythrocyte membrane proteins and/or glycoproteins, were reacted with biotinyl N-hydroxysuccinimide ester (BNHS), 3-(N-maleimido-propionyl) biocytin (MPB) or biocytin hydrazide (BCHZ), respectively. The detergent-lysed biotinylated samples were subjected to SDS-polyacrylamide gel electrophoresis, and the
Shin-Ichi Akanuma et al.
Drug metabolism and pharmacokinetics, 34(4), 239-246 (2019-06-09)
It has been known that organic anion-transporting polypeptides (Oatps) involve hepatic transports several organic anionic compounds and drugs. This study aimed to investigate sulforhodamine-101 (SR-101) distribution in the rat liver, determine the molecules responsible for the distribution, and delineate the
Diego Muñoz et al.
Cancers, 11(9) (2019-09-12)
X-linked inhibitor of apoptosis protein (XIAP) is an emerging crucial therapeutic target in cancer. We report on the discovery and characterisation of small organic molecules from Piper genus plants exhibiting XIAP antagonism, namely erioquinol, a quinol substituted in the 4-position

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