340855

Glutaraldehyde solution

50 wt. % in H₂O

• Synonym(s): Glutaric dialdehyde solution, Pentane-1,5-dial
• Linear Formula: OHC(CH₂)₃CHO
• CAS Number: 111-30-8
• Molecular Weight: 100.12
• Beilstein: 605390
• MDL number: MFCD00007025
• UNSPSC Code: 12352114
• PubChem Substance ID: 24860868
• NACRES: NA.21
• form: liquid

Properties

• vapor density: 1.05 (vs air)
• Quality Level: 200
• vapor pressure: 15 mmHg ( 20 °C)
• form: liquid
• concentration: 50 wt. % in H₂O
• technique(s): electron microscopy: suitable; immunoblotting: suitable; immunohistochemistry: suitable
• color: colorless
• refractive index: n20/D 1.42
• mp: -21 °C ((-6 °F))
• solubility: water: soluble
• density: 1.106 g/mL at 25 °C
• suitability: suitable for microfluidics/nanofluidics
• application(s): cell analysis; genomic analysis; life science and biopharma; sample preparation
• SMILES string: [H]C(CCCC([H])=O)=O
• InChI: 1S/C5H8O2/c6-4-2-1-3-5-7/h4-5H,1-3H2
• InChI key: SXRSQZLOMIGNAQ-UHFFFAOYSA-N

Description

General description

Glutaraldehyde (Glutaric dialdehyde, Pentane-1,5-dial) is an aliphatic dialdehyde that can be employed as an effective biochemical reagent for various purposes such as a protein crosslinker, enzyme immobilization in microscopy, histochemistry, and cytochemistry. It serves as an amine-reactive homobifunctional crosslinker and a cell fixative before SDS-PAGE, staining, or electron microscopy, making it suitable for a wide range of morphological studies.

Glutaraldehyde is capable of efficiently crosslinking amine and hydrazine derivatives to proteins and other amine-containing polymers. Notably, in certain reactions, biotin hydrazides have been directly coupled to nucleic acids using glutaraldehyde, presenting a potential utility in conjugating fluorescent hydrazides and hydroxylamines to DNA. In histology studies, Glutaraldehyde is also employed to preserve tissue sections and prepare them for closer examination.

Application

Cross-linking agent for gelatin,[1][2] poly(vinyl alcohol),[3] and polyheptapeptides.[4]

Glutaraldehyde may be used in the following studies:

• To compose the fixative solution (Glutaraldehyde + Paraformaldehyde + NaPO₄) for use in high-resolution light microscopy and electron microscopy studies.[5]
• To study the conjugation of goat anti-horseradish peroxidase with alkaline phosphatase by a reported method.[6]
• To compose the primary fixative, which is employed to protect the deterioration of cytoplasmic features of yeast cells during permanganate fixation.[7]

Features and Benefits

• Versatile and adaptable for wide variety of research applications
• Ready-made solution reduces the need for preparation time

Other Notes

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To gain a comprehensive understanding of our extensive range of Monosaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Safety Information

• Pictograms: GHS06,GHS08,GHS05,GHS09
• Signal Word: Danger
• Hazard Statements: H302,H314,H317,H331,H334,H335,H410
• Precautionary Statements: P273 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3
• Target Organs: Respiratory system
• Supplementary Hazards: EUH071
• Storage Class Code: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 3