About two million new cases of leishmaniasis with 50 000 associated deaths occur worldwide each year. Promastigotes of the causative Leishmania spp. develop from the procyclic stage to the highly virulent metacyclic stage within the sand fly vector. We hypothesized
Journal of pharmaceutical sciences, 101(1), 92-101 (2011-09-17)
Membrane proteins are attractive therapeutic targets, however the presence of detergents complicates biophysical binding measurements. Difficulties in determining quantitative dissociation constants for problematic membrane proteins were addressed by combining analytical ultracentrifugation and classical light scattering techniques. Validation of the algorithm
The Journal of the Acoustical Society of America, 130(5), 3482-3488 (2011-11-18)
Octyl β-D-glucopyranoside (OGP) has been reported to completely inhibit cavitation-induced cell lysis in vitro, possibly by quenching critical free-radical effects. In this study, the influence of OGP on cell lysis in a 60 rpm rotating-tube exposure apparatus was assessed. HL-60
Protein expression and purification, 86(1), 12-20 (2012-09-12)
Generating stable conditions for membrane proteins after extraction from their lipid bilayer environment is essential for subsequent characterization. Detergents are the most widely used means to obtain this stable environment; however, different types of membrane proteins have been found to
Journal of molecular modeling, 19(2), 589-599 (2012-09-14)
Density functional theory calculations on two glycosides, namely, n-octyl-β-D-glucopyranoside (C(8)O-β-Glc) and n-octyl-β-D-galactopyranoside (C(8)O-β-Gal) were performed for geometry optimization at the B3LYP/6-31G level. Both molecules are stereoisomers (epimers) differing only in the orientation of the hydroxyl group at the C4 position.
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