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S2GPU05RE

Millipore

Millipore® Stericup® Quick Release Vacuum Filtration System

Millipore® Stericup® Quick Release Vacuum Filtration System

pore size 0.22 μm, polyethersulfone membrane, funnel capacity 500 mL, pack of 12 ea

Synonym(s):

SCGPU05RE, Stericup Quick Release-GP Sterile Vacuum Filtration System, sterile vacuum filter unit, sterile vacuum filtering unit, vacuum filtration system

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About This Item

UNSPSC Code:
41104914
eCl@ss:
32031623
NACRES:
NB.24
pore size:
0.22 μm pore size
H:
263 mm
capacity:
500 mL
diam.:
12.5 cm
filter diam.:
73 mm
filtration area:
40 cm2
funnel capacity:
500 mL
packaging:
pack of 12 ea
material:
polyethersulfone membrane
polystyrene bottle
polystyrene funnel(s)
polystyrene housing
Pricing and availability is not currently available.

material

polyethersulfone membrane
polystyrene bottle
polystyrene funnel(s)
polystyrene housing

Quality Level

sterility

sterile; irradiated

product line

Stericup®

feature

hydrophilic

packaging

pack of 12 ea

parameter

45 °C max. temp.

description

2.4 mL (hold-up volume)

technique(s)

sterile filtration: suitable

H

263 mm

capacity

500 mL

diam.

12.5 cm

filter diam.

73 mm

filtration area

40 cm2

funnel capacity

500 mL

matrix

Express PLUS

pore size

0.22 μm pore size

fitting

inlet funnel
outlet flask (Receiver)

shipped in

ambient

General description

Stericup Quick Release-GP sterile vacuum filtration system combines a 500 mL Steritop bottle top filter unit, which uses a 0.22 µm pore size fast flow PES Express PLUS membrane, with a 500 mL receiver flask for sterilizing tissue culture media, protein & DNA.

Application

For Research Use Only
Stericup® and Steritop® systems are designed to reduce foaming and protein denaturation while maximizing flow and can be used for sterile vacuum filtration of aqueous solutions such as tissue culture media and biological fluids.

  • Tissue culture media +/- additives
  • Buffers
  • Biological solutions

Features and Benefits

  • The Quick Release filter funnel disconnects from the receiver bottle with just a quarter turn—reducing the likelihood of spillage and making it easier to manipulate on the bench or under the hood.
  • Bold product identification on the Steritop® filter funnel plainly displays membrane characteristics, including composition and pore size, for rapid, application-appropriate product selection.
  • Click seal cap is flanged and modified to enable a firm grip in wet or dry conditions—and a tactile stop confirms secure closure. Worry less about the possibility of spilling or contamination of sterilized contents.
  • Frosted, ample writing surface on the receiver bottle and lighter cap color facilitate clear labelling to improve legibility and reduce unreadable smears.
  • Click seal cap rests on the side to avoid risk of contamination.
  • Receiver bottles are stackable to save storage space.

Legal Information

EXPRESS PLUS is a trademark of Merck KGaA, Darmstadt, Germany
Millipore is a registered trademark of Merck KGaA, Darmstadt, Germany
Stericup is a registered trademark of Merck KGaA, Darmstadt, Germany

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Articles

Learn about the impact of filtration on hematopoietic cell culture media containing vital T cell factors and other media components.

Evaluation of sterile filtration devices for protein preservation in sterile filtration of cell culture media.

Ensure optimal cell culture outcomes with ultrapure, endotoxin-free water. Learn about the impact of water impurities on mammalian cell cultures and how to achieve optimal cell growth.

Overview on how to maintain proper cell culture laboratory safety including steps on performing lab risk assessments, surface disinfection, biohazard and waste removal. Free ECACC handbook download.

Related Content

What are you filtering out? What do you want to keep? Pick the right membrane for your filtration.

Learn how to effectively filter fetal bovine serum (FBS) using Stericup® Quick Release vacuum filtration devices, including flow test data and analysis.

This article shows that a PES membrane containing Stericup Quick Release filter provides an alternative for the pluripotent expansion of embryonic stem cells.

Questions

1–10 of 16 Questions  
  1. What is the Corning alternative for the product CLS431097, which is a Corning® bottle-top vacuum filter system with specific features like a polyethersulfone membrane, pore size of 0.22 μm, membrane area of 33.2 cm2, and a filter capacity of 500 mL?

    1 answer
    1. The recommended alternative product is S2GPU05RE, Stericup Quick Release-GP Sterile Vacuum Filtration System from Millipore. This system includes a 500 mL Steritop bottle top filter unit utilizing a 0.22 µm pore size fast flow PES Express PLUS membrane, along with a 500 mL receiver flask for the sterilization of tissue culture media, protein, and DNA.

      Helpful?

  2. What tubing can be used with Stericup® and Steritop® vacuum filters?

    1 answer
    1. Stericup® and Steritop® filters are fitted with a 1/4” tube adapter. We recommend 3/16 in. (4.8 mm) ID silicone tubing to fit this tube adapter: https://www.sigmaaldrich.com/product/mm/xx7100004

      Helpful?

  3. What is the expiration date of Stericup® and Steritop® filters?

    1 answer
    1. Typically 3 years from sterilization.

      Helpful?

  4. Can Stericup® and Steritop® filters be reused?

    1 answer
    1. All Stericup® and Steritop® filters were developed for single use, disposable processing.

      Helpful?

  5. Can Stericup® bottles be placed inside freezers for long-term storage of media?

    1 answer
    1. You can successfully freeze and store many aqueous solutions (such as culture media) in Stericup® bottles at temperatures up to -20 °C (-4 °F). It is strongly recommended that you run a sample stability trial under your actual storage conditions prior to using Stericup® bottles for frozen storage.

      Helpful?

  6. What is the chemical compatibility of the various Stericup® and Steritop® filters?

    1 answer
    1. Please refer to our guide, "Chemical compatibility of filter components": https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/302/855/membrane-chemical-compatibility-ds2532en-ms.pdf

      Helpful?

  7. What is the maximum processing volume for Stericup® and Steritop® filters? Can I add more volume after initial filtration?

    1 answer
    1. All Stericup® and Steritop® filters were developed for single-use application. The maximum volume is dependent on the receiver bottle capacity. Stericup® filters come with a receiver bottle with a specified maximum volume. For Steritop® filters, the maximum recommended volume is 2 L with an appropriate-sized receiver bottle. Larger processing volumes can result in membrane fouling, increase chance of spillage and contamination, and lead to possible airlock*.
      *Note that devices with PES membranes can airlock if filtered to dryness. Some liquid must be left on top of the filter, then the system stopped, receiver changed or emptied, and more sample volume added before restart. This increases the chance of spillage.

      Helpful?

  8. Can we filter oil-type liquids with Stericup® filters?

    1 answer
    1. Likely not, due to pore size/possible fouling/chemical compatibility. Refer to our guide, "Chemical compatibility of filter components": https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/302/855/membrane-chemical-compatibility-ds2532en-ms.pdf

      Helpful?

  9. What Bacterial Endotoxins Test is used?

    1 answer
    1. The test method used complies with USP 85 and EP 2.6.14 to ensure the absence of endotoxin in our products.

      Helpful?

  10. Which type of pump should be used with Stericup® and Steritop® vacuum filters?

    1 answer
    1. We recommend our Chemical Duty Pump: https://www.sigmaaldrich.com/product/mm/wp6111560

      Helpful?

1–10 of 16 Questions  

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