CBA059
Annexin V-FITC Apoptosis Detection Kit II
fluorometric assay kit for measuring Annexin V in apoptotic cells.
Synonym(s):
Annexin V Assay, Apoptosis Detection Kit
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About This Item
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Quality Level
usage
sufficient for 20 tests
packaging
pkg of 1 kit
manufacturer/tradename
Calbiochem®
technique(s)
flow cytometry: suitable
immunofluorescence: suitable
detection method
fluorometric
shipped in
wet ice
storage temp.
2-8°C
General description
A convenient kit for the identification of changes in the plasma membrane that occur during apoptosis. In apoptotic cells, phosphoatidylserine (PS) is translocated from the inner to the outer leaflet of the plasma membrane, allowing for the detection of PS on the cell surface. In the presence of Ca 2+, Annexin V exhibits a high affinity for PS and binds cells with exposed PS. Annexin V, when conjugated to a fluorophore, can be used to monitor apoptopic cells using flow cytometry or immunofluorescence microscopy.
Components
Kit contains:
- Annexin V-FITC
- Binding Buffer
- Propidium Iodide
- user protocol.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
10 - Combustible liquids
Certificates of Analysis (COA)
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