ABT95
Anti-TGN46, Antibody
from rabbit, purified by affinity chromatography
Synonym(s):
Trans-Golgi network integral membrane protein 2, TGN38 homolog, TGN46, TGN48, Trans-Golgi network protein TGN51
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, rat, human
species reactivity (predicted by homology)
canine (based on 100% sequence homology)
technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... TGOLN2(10618)
General description
TGN46 is one of several proteins that utilize retrograde transport to travel between the trans-Golgi network (TGN) and the endocytic pathway. Retrograde transport involves the tethering, docking, fusion, and post translational modification of TGN proteins. Further studies are still required to elucidate the roles and mode of action for TGN46.
Specificity
This antibody recognizes the cytoplasmic domain of TGN46.
Immunogen
Epitope: Cytoplasmic domain
KLH-conjugated linear peptide corresponding to the cytoplasmic domain of human TGN46.
Application
Anti-TGN46 detects levels of TGN46 proteins & has been published & validated for use in wb, IP & ICC.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Vesicular Trafficking
Vesicular Trafficking
Western Blot Analysis: 2 µg/mL of this antibody detected TGN46 in 10 µg of NIH/3T3 cell lysate. Multiple uncharacterized bands may be observed in some cell lysates.
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected TGN46 in A431 and HeLa cells.
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected TGN46 in A431 and HeLa cells.
Quality
Evaluated by Immunoprecipitation in HeLa cell lysate.
Immunoprecipitation Analysis: 0.2 µg of this antibody immunoprecipitated TGN46 in 500 µg of HeLa cell lysate (An uncharacterized band at ~29 kDa may be observed in some cell lysates.).
Immunoprecipitation Analysis: 0.2 µg of this antibody immunoprecipitated TGN46 in 500 µg of HeLa cell lysate (An uncharacterized band at ~29 kDa may be observed in some cell lysates.).
Target description
~110 kDa observed. This protein is observed at ~110 kDa, and it is subject to glycosylation (Khanobdee, K. et al. (2004). Mol Vis. 13(10):933-42.; Prescott, A. R., et al. (1997). Eur J Cell Biol. 72(3):238-246.).
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
HeLa cell lysate
HeLa cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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