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553571

Sigma-Aldrich

Anti-Ras (31-43) Rabbit pAb

liquid, Calbiochem®

Synonym(s):

Anti-Ras GDP/GTP Binding Protein

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody product type

primary antibodies

clone

polyclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

rat, bovine, human, mouse

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... RAB2A(5862)

General description

Immunoaffinity rabbit polyclonal antibody. Recognizes all forms of wild type Ras and the mutant G12V Ras protein.
Recognizes all forms of wild type Ras, including the mutant G12V Ras protein.
This Anti-Ras (31-43) Rabbit pAb is validated for use in Immunoblotting for the detection of Ras (31-43).

Immunogen

a synthetic peptide [EYDPTIEDSYRKQ(C)] corresponding to amino acids 31-43 in the effector binding Loop (L2) of human H-, K-, and N-Ras, conjugated to KLH

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Analysis Note

Positive Control
Rat brain tissue

Other Notes

By immmunoblotting, this anti-pan-Ras polyclonal antibody reacts strongly with recombinant Ras fusion protein as well as with recombinant glutathione S-transferase (GST) human Ras and [G12V]GST human Ras. Does not cross-react with members of the Rho or Rab families of Ras-related small G-proteins. No specific reactivity is detected by immunoblotting with rat, mouse or human cell lines or tissue extracts. Variables associated with assay conditions will dictate the proper working dilution.
Hardie, G. and Hanks, S. 1995. in The Protein Kinase Facts Book, Protein-Serine Kinases p. 418,Academic Press, San Diego, CA.
Sawada, M., et al. 1989. Nucleic Acid Res.17, 8867.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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