539508

PP2A₁, Bovine Kidney

• Synonym(s): Protein Phosphatase 2A₁
• Enzyme Commission number: 3.1.3.16 (BRENDA, IUBMB)
• UNSPSC Code: 12352202

Properties

• Assay: ≥90% (SDS-PAGE)
• Quality Level: 100
• form: liquid
• specific activity: ≥750 units/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Native, PP2A₁ from bovine kidney. A divalent cation-independent protein serine/threonine phosphatase involved in the control of numerous cellular processes. Has a catalytic subunit of 36 kDa, a subunit of 55 kDa, and a regulatory subunit of 60 kDa. Reported to associate with microtubules and to bind to purified tubulin polymerized by TAXOL^® Drug.

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will release 1.0 nmol of [³²P]P_i from ³²P-labeled phosphorylase per min at 30°C, pH 7.0.

Physical form

In 50 mM Tris-HCl, 14 mM β-ME, 1 mM benzamidine, 1 mM EDTA, 100 µM PMSF, 50% glycerol, pH 7.0.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

Hiraga, A. and Tamura, S. 2000. Biochem. J.346, 433.
Ferrigno, P., et al. 1993. Mol. Biol. Cell. 4, 669.
Guo, H. and Damuni, Z. 1993. Proc. Natl. Acad. Sci. USA90, 2500.
Hendrix, P., et al. 1993. J. Biol. Chem.268, 15267.
Amick, G.D., et al. 1992. Biochem. J. 287, 1019.
Cohen, P., et al. 1990. Trends Biochem. Sci.15, 98.
Damuni, Z., et al. 1989. J. Biol. Chem.264, 6412.1

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Taxol is a registered trademark of Bristol-Myers Squibb Co.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable