219489

Ceramide Kinase Inhibitor, K1

• Synonym(s): Ceramide Kinase Inhibitor, K1, F-12509A Cyclic Product K1
• Empirical Formula (Hill Notation): C₂₁H₂₈O₄
• CAS Number: 1258005-85-4
• Molecular Weight: 344.44
• UNSPSC Code: 12352200

Properties

• Assay: ≥95% (mixture of enantiomers, NMR)
• Quality Level: 100
• form: powder
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: yellow
• solubility: ethanol: 20 mg/mL
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

This product has been discontinued.

A cell-permeable tetracyclic quinone compound that acts as a specific, reversible, and non-competitive inhibitor of CerK (ceramide kinase) activity (IC₅₀ = ~5 µM) with little effect against SPHK1/2 or DGKγ (IC₅₀ >100 µM). Shown to reduce cellular C1P (ceramide-1-phosphate) synthesis by 40% at ≥ 20 µM in a rat basophilic leukemia cell line RBL-2H3 and block CerK-mediated degranulation in both RBL-2H3 and murine BMMC (bone marrow-derived mast cells) in a dose-dependent manner. Exhibits no cytotoxic effect against RBL-2H3 even at concentrations as high as 100 µM.

A cell-permeable tetracyclic quinone compound that acts as a specific, reversible, and non-competitive inhibitor of CerK (ceramide kinase) activity (IC₅₀ ~5 µM) with little effect against SPHK1/2 or DGKγ (IC₅₀ >100 µM). Shown to reduce cellular C1P (ceramide-1-phosphate) synthesis by 40% at ≥20 µM in a rat basophilic leukemia cell line RBL-2H3 and block CerK-mediated degranulation in both RBL-2H3 and murine BMMC (bone marrow-derived mast cells) in a dose-dependent manner. Exhibits no cytotoxic effect against RBL-2H3 even at concentrations as high as 100 µM.

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Kim, J.W., et al. 2007. Biochim. Biophys. Acta1771, 1262.
Kumada, H., et al. 2007. Biosci. Biotechnol. Biochem.71, 2581
Kim, J.W., et al. 2005. Biochim. Biophys. Acta1738, 82.

Legal Information

Sold for research purposes only.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10-13 - German Storage Class 10 to 13