14-787-M

PI3 Kinase (p110α (H1047R)/p85α) Murine, 10 µg

Complex of N-terminal 6His-tagged, recombinant, full-length mouse p110α containing the mutation H1047R α & untagged, recombinant, full length mouse p85α. For use in Kinase Assays.

• UNSPSC Code: 12352200
• eCl@ss: 32160405
• NACRES: NA.32

Properties

• biological source: mouse
• Quality Level: 100; 300
• recombinant: expressed in baculovirus infected Sf21 cells
• mol wt: M_w 129 kDa (p110α; H1047R); M_w 83.6 kDa (p85α)
• manufacturer/tradename: Upstate^®
• technique(s): activity assay: suitable (kinase)
• NCBI accession no.: NM_006218.2
• UniProt accession no.: P42336
• Gene Information: mouse ... PIK3CA(18706)

Description

General description

Complex of N-terminal 6His-tagged, recombinant, full-length mouse p110α containing the mutation H1047R αand untagged, recombinant, full length mouse p85α.

Product Source: Coexpressed by baculovirus in Sf21 insect cells.

The H1047R substitution in p110α is associated with a number of human cancers. The residue is conserved between the human and mouse sequences. In vitro studies have shown that this mutation confers increased lipid kinase activity compared to wild type, and is able to induce oncogenic transformation.

Application

Research Category
Inflammation & Immunology

Biochem/physiol Actions

Protein Target: PI3Kα/p85α

Target Sub-Family: PI3K/PI4K

Packaging

Also available in 250μg size --call for pricing and availability and reference catalog number 14-787M when ordering the 250μg size.

Physical form

Ni²⁺/NTA-agarose

Storage and Stability

Store at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H317
• Precautionary Statements: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable