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Sigma-Aldrich

Alcalase® Enzyme, Bacillus licheniformis

Synonym(s):

Protease from Bacillus licheniformis, subtilisin A

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About This Item

Enzyme Commission number:
3.4.21.14 (BRENDA, IUBMB)
UNSPSC Code:
12352202
NACRES:
NA.56

biological source

Bacillus licheniformis

Quality Level

100

form

liquid

specific activity

≥0.75 Anson units/mL

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

technique(s)

activity assay: suitable

color

dark brown

application(s)

life science and biopharma

shipped in

ambient

storage temp.

2-8°C

General description

Alcalase® is a serine endopeptidase that consists primarily of subtilisin A.

Application

Alcalase® Enzyme, Bacillus licheniformis has been used in the proteolytic digestion and extraction of polysaccharides in various sponge species. It has also been used as an endoprotease enzyme in enzymatic hydrolysis for the production of fish protein hydrolysate.

Biochem/physiol Actions

Alcalase® is suitable for the hydrolysis of proteins with an optimal pH of 7.0-8.5. It is used as an additive in detergent, dehairing, and bating leather. Alcalase is also used in meat tenderizing, cheese flavor development, baked manufacture, and improving the digestibility of animal feeds. Commercial Alcalase® comprises a liquid enzymatic preparation containing 50% glycerol, 41% water, and 9% protease extract from Bacillus licheniformis.

Warning

Toxicity: Harmful (C)

Unit Definition

One unit is defined as the amount of enzyme that will release 1.0 µmol L-tyrosine from hemoglobin per min at 25°C, pH 7.5.

Reconstitution

Please refer to the vial label for lot-specific density.

Legal Information

Alcalase is a registered trademark of Novozymes Corp.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Health hazardCorrosion
GHS08,GHS05

Signal Word

Danger

Hazard Statements

H318,H334,H412

Hazard Classifications

Aquatic Chronic 3 - Eye Dam. 1 - Resp. Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Fish protein hydrolysate production by acid and enzymatic hydrolysis
Wisuthiphaet N, et al.
Applied Science and Engineering Progress, 9(4) (2016)
Monitoring of sulfated polysaccharide content in marine sponges by Raman spectroscopy
Maia LF, et al.
Vibrational Spectroscopy, 87, 149-156 (2016)
Veymar G Tacias-Pascacio et al.
International journal of biological macromolecules, 165(Pt B), 2143-2196 (2020-10-23)
This review aims to cover the uses of the commercially available protease Alcalase in the production of biologically active peptides since 2010. Immobilization of Alcalase has also been reviewed, as immobilization of the enzyme may improve the final reaction design

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