Precoated Kieselguhr or mixed Silica gel/Kieselguhr plates are only of small importance nowadays. Kieselguhr layers are used like cellulose layers for the TLC separation of polar and moderately polar substances. Polyamide, on the other hand , is used for the separation of flavonoids and other phenolic substances.
Linkage
Replaces: 5567-7; 5567
Analysis Note
Layer thickness: 170 - 220 µm Deviation of layer thickness per plate: ≤ 30 µm Chromatografic testings: A) colour test hRf-values - bleu vif organol, colour test, lipophile: 20 - 30 - Ceres Black G, colour test, lipophile: 50 - 60 - ceres Violet brn, colour test, lipophile: 60 - 70 Typical value determined on a conditioned sheet Eluent: toluene (45% rel. humidity) B) PHB- Ester - phb-methylester, phb-ester-test: 20 - 30 - phb-ethylester, phb-ester-test: 30 - 40 - phb-propylester, phb-ester-test: 40 - 50 Typical value determined on a sheet Eluent: Pentane/ glacial acetic acid (88/12 v/v)
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