07-2268
Anti-phospho-TBC1D1 Antibody (Ser237)
from rabbit, purified by affinity chromatography
Synonym(s):
TBC1 domain family member 1, TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
Recommended Products
biological source
rabbit
Quality Level
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human
species reactivity (predicted by homology)
ox (based on 100% sequence homology), chimpanzee (based on 100% sequence homology), mouse (based on 100% sequence homology), rat (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology)
technique(s)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
phosphorylation (pSer237)
Gene Information
human ... TBC1D1(23216)
General description
TBC1D1 is the first member of a family of proteins that contain the tbc box motif of 180-220 amino acids and may be related to cell growth and differentiation. The composition of TBC1D1 has the same function as a known regulator of insulin-mediated Glut4 translocation and may play a role in severe human obesity. An insulin-independent increase in glucose transport several hours after exercise has been attributed to TBC1D1 phosphorylation. The R125W mutation of TBC1D1 is thought to decrease skeletal muscle glucose transport and could be the main reason for the obesity that seems to be linked to this type of mutation.
Specificity
This antibody recognizes TBC1D1 phosphorylated at Ser237.
Immunogen
Epitope: Phosphorylated Ser237
KLH-conjugated linear peptide corresponding to human TBC1D1 phosphorylated at Ser237.
Application
Research Category
Signaling
Signaling
Research Sub Category
Insulin/Energy Signaling
Glucose/Glycogen Metabolism
Insulin/Energy Signaling
Glucose/Glycogen Metabolism
This phospho-TBC1D1 antibody is validated for use in WB for the detection of the phospho-TBC1D1 protein.
Quality
Evaluated by Western Blot in lambda phosphatase treated and untreated HEK293 cell lysates.
Western Blot Analysis: 1 µg/mL of this antibody detected TBC1D1 on 10 µg of lambda phosphatase treated and untreated HEK293 cell lysates.
Western Blot Analysis: 1 µg/mL of this antibody detected TBC1D1 on 10 µg of lambda phosphatase treated and untreated HEK293 cell lysates.
Target description
~145 kDa observed
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Lambda phosphatase treated and untreated HEK293 cell lysates
Lambda phosphatase treated and untreated HEK293 cell lysates
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Marie Locard-Paulet et al.
Molecular systems biology, 16(7), e9524-e9524 (2020-07-04)
T-cell receptor (TCR) ligation-mediated protein phosphorylation regulates the activation, cellular responses, and fates of T cells. Here, we used time-resolved high-resolution phosphoproteomics to identify, quantify, and characterize the phosphorylation dynamics of thousands of phosphorylation sites in primary T cells during
Sharon C Hook et al.
Scientific reports, 10(1), 17953-17953 (2020-10-23)
Proteins involved in the spaciotemporal regulation of GLUT4 trafficking represent potential therapeutic targets for the treatment of insulin resistance and type 2 diabetes. A key regulator of insulin- and exercise-stimulated glucose uptake and GLUT4 trafficking is TBC1D1. This study aimed
Jonas Roland Knudsen et al.
Experimental physiology, 104(5), 704-714 (2019-02-03)
What is the central question of this study? Resolving the mechanism(s) leading to glucose transporter 4 (GLUT4) translocation to the muscle surface membrane has great therapeutic potential. However, the measurement of GLUT4 translocation is technically challenging. Here, we asked whether
Yang Liu et al.
Nature, 578(7796), 605-609 (2020-02-14)
The activation of adenosine monophosphate-activated protein kinase (AMPK) in skeletal muscle coordinates systemic metabolic responses to exercise1. Autophagy-a lysosomal degradation pathway that maintains cellular homeostasis2-is upregulated during exercise, and a core autophagy protein, beclin 1, is required for AMPK activation in
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