330687
Avanti
Cer1P ELISA (Clear)
Avanti Research™ - A Croda Brand
Synonym(s):
Cer1P ELISA, C16 Ceramide-1-Phosphate ELISA Snoopers™, Clear, for use in absorbance applications
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About This Item
UNSPSC Code:
41116158
NACRES:
NA.84
form
liquid
manufacturer/tradename
Avanti Research™ - A Croda Brand
packaging
1 ea of 1 × (ELISA strip packaged in foil pouch)
shipped in
wet ice
storage temp.
2-8°C
General description
Avanti Lipid Snoopers™ are lipid microarrays useful in semi-quantitative measurements. These snoopers are relatively stable and provide a higher sensitivity compared to the conventional enzyme-linked immunosorbent assays (ELISA) methods. Ceramide-1-Phosphate (Cer1P) is a bioactive anionic sphingolipid. Ceramide kinase (CERK) mediates the synthesis of Cer1P. Cer1P is also synthesized by the hydrolysis of sphingomyelin in the presence of enzyme sphingomyelinase (SMase).
ELISA Snoopers™ are 8-well strips pre-coated with a single high-quality lipid species. They can be used to investigate protein-lipid interactions, and characterize and titrate lipid binding proteins. This format provides the following advantages:
The 8-well strips are available in optically clear, white, or black surfaces for use in colormetric, luminescent and fluorescent assays, respectively. Avanti Lipid Snoopers ELISA Strips allow multiple sample conditions to be probed simultaneously in a single plate making them a perfect complement to Avanti Lipid Snoopers Nitrocellulose Strips.
- quantitative lipid-protein binding studies
- assemble a 96-well plate using only the lipids of interest for binding studies
- flexibility - use 8 to 96 wells, and 1 to 12 lipids as needed.
The 8-well strips are available in optically clear, white, or black surfaces for use in colormetric, luminescent and fluorescent assays, respectively. Avanti Lipid Snoopers ELISA Strips allow multiple sample conditions to be probed simultaneously in a single plate making them a perfect complement to Avanti Lipid Snoopers Nitrocellulose Strips.
Biochem/physiol Actions
Ceramide-1-Phosphate (Cer1P) mediates macrophage migration, regulates autocrine and paracrine mediators. Elevated levels of Cer1P leads to negative regulation of ceramide kinase (CERK). The intracellular calcium levels modulate Cer1P and CERK functionality. Cer1P is also a crucial regulator of phagocytosis, inflammation, apoptosis and mitosis. Avanti Lipid Snoopers™ have been used successfully in elucidating the role of annexin V in membrane trafficking.
Packaging
ELISA Strip Packaged in Foil Pouch (330687-1EA)
Legal Information
Avanti Research is a trademark of Avanti Polar Lipids, LLC
Snoopers is a trademark of Avanti Polar Lipids, LLC
related product
Product No.
Description
Pricing
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
No data available
Flash Point(C)
No data available
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
A Nonradioactive Fluorimetric SPE-Based Ceramide Kinase Assay Using NBD-C6-Ceramide
Van O, et al.
Journal of Lipids, 2012 (2012)
Ceramide-1-phosphate, a new bioactive sphingolipid in regulating cell signaling
Marcu AC and Chalfant CE
Future Lipidology , 2(1), 75-84 (2007)
Ceramide 1-phosphate mediates endothelial cell invasion via the annexin a2-p11 heterotetrameric protein complex
Hankins JL, et al.
Test, 288(27), 19726-19738 (2013)
Regulation of the amount of ceramide-1-phosphate synthesized in differentiated human podocytes
Mallela SK, et al.
Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, 1864(12), 158517-158517 (2019)
Avanti lipid tools: connecting lipids, technology, and cell biology
Sims KH, et al.
Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, 1841(8), 1038-1048 (2014)
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