B6916
Bradford Reagent
for 0.1-1.4 mg/ml protein
Synonym(s):
Coomassie dye binding protein assay, Coomassie dye binding protein assay, Protein dye reagent, Protein dye reagent
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General description
Application
Features and Benefits
- The reagent is ready to use. No mixing or dilution required.
- Color development is rapid. Only a five minute incubation and then the sample is read a 595 nm.
- Reducing sugars and reducing substances along with thiols do not interfere with this reagent.
- Reagent is suitable for micro (1-10 μg/ml) and standard (50-1400 μg/ml) assays.
- Can be used in microwell plate assays.
- Inexpensive assay.
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Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 2
Target Organs
Eyes,Central nervous system
Storage Class Code
8B - Non-combustible corrosive hazardous materials
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
The field of proteomics is continually looking for new ways to investigate protein dynamics within complex biological samples. Recently, many researchers have begun to use RNA interference (RNAi) as a method of manipulating protein levels within their samples, but the ability to accurately determine these protein amounts remains a challenge. Fortunately, over the past decade, the field of proteomics has witnessed significant advances in the area of mass spectrometry. These advances, both in instrumentation and methodology, are providing researchers with sensitive assays for both identification and quantification of proteins within complex samples. This discussion will highlight some of these methodologies, namely the use of Multiple Reaction Monitoring (MRM) and Protein-AQUA.
Protocols
To determine protein content, the Warburg-Christian method refers to measuring protein samples at 280 nm using a spectrophotometer.
Rules and good practice in sample preparation for Western blot sample preparation from cell culture and tissue samples.
Related Content
Protein quantification methods, reagents, and immunoassay technology for accurately measuring the protein concentrations in a variety of samples.
Products for traditional and alternative protein quantitation techniques available, including BCA, Bradford, Lowry, and more.
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