SCR150
AldeRed® ALDH Detection Assay
Synonym(s):
ALDH Assay, Aldehyde Dehydrogenase Assay, Cancer Stem Cell Assay
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.84
eCl@ss:
32161000
Pricing and availability is not currently available.
Quality Level
technique(s)
cell culture | stem cell: suitable, flow cytometry: suitable
fluorescence
λex 488 nm, λex 532 nm, λem 615 nm
detection method
fluorometric
shipped in
wet ice
storage temp.
2-8°C
General description
The AldeRed ALDH Detection Kit provides cancer and stem cell scientists with new capabilities for live cell isolation and characterization. The AldeRed reagent is a red-shifted fluorescent substrate for aldehyde dehydrogenase (ALDH), allowing cells to be identified and isolated by flow cytometry with concurrent use of green fluorescent cell lines, antibodies, transgenic animals and reporter assays.
Learn more about the AldeRed in Nature Communications! (Learn Now!)
High ALDH activity serves as a universal marker of stem cells, both normal and malignant. Cells can be identified and isolated based upon the enzymatic activity of ALDH, a detoxifying enzyme responsible for oxidation of hazardous aldehyde byproducts. The marker ALDH has been used to isolate cancer stem cells from various human malignancies including bladder, breast, cervical, colon, head and neck, liver, lung, pancreas, prostate and ovary. The AldeRed assay employs a fluorescent and non-toxic ALDH substrate (AldeRed 588-A) that diffuses freely into intact and viable cells, but remains trapped inside the cells once converted by ALDH into the corresponding acid. The amount of fluorescence produced is proportional to the ALDH activity in the cells and is measured by flow cytometry, allowing fluorescence-activated cell sorting (FACS). This kit supplies the ALDH inhibitor diethylaminobenzaldehyde (DEAB), which is used in negative control testing necessary for background fluorescence assessement.
Learn more about the AldeRed in Nature Communications! (Learn Now!)
High ALDH activity serves as a universal marker of stem cells, both normal and malignant. Cells can be identified and isolated based upon the enzymatic activity of ALDH, a detoxifying enzyme responsible for oxidation of hazardous aldehyde byproducts. The marker ALDH has been used to isolate cancer stem cells from various human malignancies including bladder, breast, cervical, colon, head and neck, liver, lung, pancreas, prostate and ovary. The AldeRed assay employs a fluorescent and non-toxic ALDH substrate (AldeRed 588-A) that diffuses freely into intact and viable cells, but remains trapped inside the cells once converted by ALDH into the corresponding acid. The amount of fluorescence produced is proportional to the ALDH activity in the cells and is measured by flow cytometry, allowing fluorescence-activated cell sorting (FACS). This kit supplies the ALDH inhibitor diethylaminobenzaldehyde (DEAB), which is used in negative control testing necessary for background fluorescence assessement.
Application
• For optimal excitation and emission of AldeRed 588-A, use a combination of blue laser (488 nm) or green laser (532 nm) with PE-Texas Red detector (615 nm). Detection in proximal measurement channels (e.g. PE) is also possible, but may affect delta MFI (mean fluorescence intensity) between Test sample and DEAB-treated control sample.
• All live intact cells (even with low ALDH expression) become fluorescent upon exposure to activated AldeRed 588-A. The generation of a DEAB-treated control is necessary for each cell sample to establish baseline fluorescence.
• Handle all reagents using aseptic techniques.
• All live intact cells (even with low ALDH expression) become fluorescent upon exposure to activated AldeRed 588-A. The generation of a DEAB-treated control is necessary for each cell sample to establish baseline fluorescence.
• Handle all reagents using aseptic techniques.
Research Category
Stem Cell Research
Apoptosis & Cancer
Stem Cell Research
Apoptosis & Cancer
Preparation Note
• Store kit at 2-8°C.
• Reconstituted and activated AldeRed Reagent may be stored in aliquots at -20°C protected from light for up to 1 year. Repeated freezing and thawing is not recommended.
• AldeRed Assay Buffer supplemented with Verapamil may be stored at 2-8°C in aliquots for up to 3 months.
• Reconstituted and activated AldeRed Reagent may be stored in aliquots at -20°C protected from light for up to 1 year. Repeated freezing and thawing is not recommended.
• AldeRed Assay Buffer supplemented with Verapamil may be stored at 2-8°C in aliquots for up to 3 months.
Analysis Note
• Each lot is tested by measuring ALDH activity in SK-BR-3 cells by flow cytometry.
• AldeRed® Reagent is 95% pure (PMR)
• AldeRed® Reagent is 95% pure (PMR)
Other Notes
1. AldeRed Reagent: (Part No. CS216596). One (1) vial containing 50 µg of AldeRed reagent powder.
2. DEAB Reagent: (Part No. CS216595). One (1) vial containing 1 mL of DEAB (Diethylaminobenzaldehyde) reagent.
3. Hydrochloric Acid (2N): (Part No. CS216594). One (1) vial containing 1.5 mL of 2 Normal hydrochloric acid.
4. Dimethylsulphoxide: (Part No. CS216593). One (1) vial containing 1.5 mL of Dimethylsulphoxide (DMSO).
5. AldeRed Assay Buffer: (Part No. CS216592). Four (4) bottles containing 25 mL of AldeRed Assay Buffer each.
6. Verapamil: (Part No. CS220012). Four (4) vials containing 615 µg Verapamil powder each.
2. DEAB Reagent: (Part No. CS216595). One (1) vial containing 1 mL of DEAB (Diethylaminobenzaldehyde) reagent.
3. Hydrochloric Acid (2N): (Part No. CS216594). One (1) vial containing 1.5 mL of 2 Normal hydrochloric acid.
4. Dimethylsulphoxide: (Part No. CS216593). One (1) vial containing 1.5 mL of Dimethylsulphoxide (DMSO).
5. AldeRed Assay Buffer: (Part No. CS216592). Four (4) bottles containing 25 mL of AldeRed Assay Buffer each.
6. Verapamil: (Part No. CS220012). Four (4) vials containing 615 µg Verapamil powder each.
Legal Information
ALDERED is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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This Item | |||
|---|---|---|---|
| technique(s) cell culture | stem cell: suitable, flow cytometry: suitable | technique(s) protein quantification: suitable | technique(s) cell culture | stem cell: suitable | technique(s) protein quantification: suitable |
| detection method fluorometric | detection method colorimetric | detection method - | detection method colorimetric |
| fluorescence λex 488 nm, λem 615 nm, λex 532 nm | fluorescence - | fluorescence - | fluorescence - |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
| shipped in wet ice | shipped in - | shipped in wet ice | shipped in - |
| Quality Level 100 | Quality Level 200 | Quality Level 100 | Quality Level - |
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1
Storage Class
3 - Flammable liquids
flash_point_f
55.4 °F
flash_point_c
13 °C
wgk
WGK 3
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Protocols
分步腫瘤球形成方案，可用於產生 3D 癌症幹細胞球體。
Step-by-step tumorpshere formation protocol that can used to generate 3D cancer stem cell spheroids.
Articles
AldeRed™ 588-A 可檢測癌症幹細胞中的 ALDH 活性，有助於癌症研究。
癌症幹細胞培養基、球形板和癌症幹細胞標記，以培養和表徵 CSC 群。
閱讀幹細胞的類型，包括多能幹細胞、多能幹細胞和 iPSCs，以及它們在基礎幹細胞研究、幹細胞治療和疾病模型中的應用
Related Content
Cancer Stem Cell Proliferation in Human Prostate and Breast Cancer Cell Lines Utilizing a New Defined Serum-Free 3D Spheroid Media
Hybrid clone cells derived from human breast epithelial cells and human breast cancer cells exhibit properties of cancer stem/initiating cells.
Gauck, D; Keil, S; Niggemann, B; Zanker, KS; Dittmar, T
BMC Cancer null
Loren E Stallcop et al.
Lab on a chip, 18(3), 451-462 (2018-01-11)
Tape-based razor-printing is a flexible and affordable ultra-rapid prototyping approach for microscale device fabrication. However, integration of this prototyping approach into cell-based assay development has been limited to proof of principle demonstrations. This is in large part due to lack
Natalia Pajuelo-Lozano et al.
Theranostics, 10(21), 9601-9618 (2020-08-31)
Rationale: Gastric cancer (GC) is a solid tumor that contains subpopulations of cancer stem cells (CSCs), which are considered drivers of tumor initiation and metastasis; responsible for therapeutic resistance; and promoters of tumor relapse. The balance between symmetric and asymmetric