This product is a stabilized solution in Tris buffer, pH 7.3.
G0774
Glycerokinase from Bacillus stearothermophilus
buffered aqueous solution, ≥75 units/mg protein (biuret)
Synonym(s):
ATP:glycerol 3-phosphotransferase, Glycerol Kinase
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About This Item
CAS Number:
UNSPSC Code:
12352204
eCl@ss:
32160410
EC Number:
232-862-0
NACRES:
NA.54
MDL number:
Specific activity:
≥75 units/mg protein (biuret)
Assay:
≥5.0 mg protein/mL (biuret)
Biological source:
Bacillus sp. (Bacillus steaarothermophilus)
Pricing and availability is not currently available.
biological source
Bacillus sp. (Bacillus steaarothermophilus)
Quality Segment
assay
≥5.0 mg protein/mL (biuret)
form
buffered aqueous solution
specific activity
≥75 units/mg protein (biuret)
storage condition
dry at room temperature
color
beige
application(s)
life science and biopharma
storage temp.
2-8°C
Gene Information
Bacillus sp. ... glpK(89613566)
General description
Research area: Cell Signaling
Glycerol kinase is encoded by the GK gene on chromosome Xp21.2. Glycerol kinase is predominantly active in the liver.
Glycerol kinase is encoded by the GK gene on chromosome Xp21.2. Glycerol kinase is predominantly active in the liver.
Application
Glycerokinase from Bacillus stearothermophilus has been used in steady-state kinetics studies.[1]
Biochem/physiol Actions
Glycerol kinase catalyzes tge MgATP-dependent phosphorylation of glycerol to produce sn-glycerol-3-phosphate and is the rate limiting enzyme in the utilization of glycerol.[2][3] It is also subject to feedback regulation by fructose-1,6-bisphosphate.[4]Oxidized glycerol-3-phosphate results in dihydroxyacetone phosphate which enters either glycolysis or gluconeogenesis. Elevated glycerol is observed in glycerol kinase deficiency leading to pseudohypertriglyceridemia.
Physical form
Stabilized solution in Tris buffer, pH 7.3
Other Notes
One unit will convert 1.0 μmole of glycerol and ATP to L-α-glycerophosphate and ADP per min at pH 9.8 at 25 °C in a coupled system with PK/LDH.
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This Item | |||
|---|---|---|---|
| specific activity ≥75 units/mg protein (biuret) | specific activity 40-100 units/mg protein | specific activity - | specific activity 25-75 units/mg protein |
| Gene Information Bacillus sp. ... glpK(89613566) | Gene Information - | Gene Information - | Gene Information - |
| assay ≥5.0 mg protein/mL (biuret) | assay - | assay - | assay - |
| biological source Bacillus sp. (Bacillus steaarothermophilus) | biological source Escherichia coli | biological source Escherichia coli | biological source - |
| application(s) life science and biopharma | application(s) - | application(s) - | application(s) - |
| form buffered aqueous solution | form lyophilized powder | form ammonium sulfate suspension | form lyophilized powder |
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Related Content
Global Trade Item Number
| SKU | GTIN |
|---|---|
| G0774-500UN | 04061832923970 |
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Hello What is the composition of the "Buffered Aqueous Solution" for the G0774--Sigma-Aldrich. Glycerokinase from Bacillus stearothermophilus. This information is important and necessary for our work. Thanks
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