G6142
Glycerokinase from Cellulomonas sp.
lyophilized powder, 25-75 units/mg protein
Synonym(s):
glpK, ATP:glycerol 3-phosphotransferase, Glycerol Kinase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-862-0
MDL number:
eCl@ss:
32160410
Specific activity:
25-75 units/mg protein
form
lyophilized powder
Quality Segment
specific activity
25-75 units/mg protein
mol wt
~128 kDa (by gel filtration)
composition
Protein, ≥60% biuret
storage temp.
−20°C
General description
Research area: Cell Signaling
Glycerol kinase (GK) is part of the FGGY carbohydrate kinase family.
Glycerol kinase (GK) is part of the FGGY carbohydrate kinase family.
Isoelectric point : 4.2
Michaelis constants : 4.4 x 10-5M (Glycerol), 4.3 x 10-4M (ATP)
Inhibitors : p-Chloromercuribenzoate, heavy metal ions (Pb++, Fe++, Hg++, Ag+)
Optimum pH : 9.8 (G-3-PDH system), 7.8 (G-3-P oxidase system) Optimum temperature : 500C
pH Stability : pH 5.5 x 10.0 (25oC, 20hr)
Thermal stability : below 40oC (pH 7.5, 15min)
Substrate specificity : This enzyme catalyzes the stereospecific transfer of the terminal
phosphoryl moiety of ATP to one of the primary hydroxyl group of
glycerol, forming sn-glycerol-3-P. The enzyme has the highest
specificity for glycerol, and also phosphorylates dihydroxyacetone
and glyceraldehyde (Table 1,2). Mg++ is essentially required for the
reaction.
Michaelis constants : 4.4 x 10-5M (Glycerol), 4.3 x 10-4M (ATP)
Inhibitors : p-Chloromercuribenzoate, heavy metal ions (Pb++, Fe++, Hg++, Ag+)
Optimum pH : 9.8 (G-3-PDH system), 7.8 (G-3-P oxidase system) Optimum temperature : 500C
pH Stability : pH 5.5 x 10.0 (25oC, 20hr)
Thermal stability : below 40oC (pH 7.5, 15min)
Substrate specificity : This enzyme catalyzes the stereospecific transfer of the terminal
phosphoryl moiety of ATP to one of the primary hydroxyl group of
glycerol, forming sn-glycerol-3-P. The enzyme has the highest
specificity for glycerol, and also phosphorylates dihydroxyacetone
and glyceraldehyde (Table 1,2). Mg++ is essentially required for the
reaction.
Application
Glycerokinase from Cellulomonas sp. has been used:
- for determining the kinetic characteristics of human and trypanosomatid phosphofructokinases using an enzyme-linked kinetic assay.
- to study the effect of sugar in fluorescence emission.
- in 2-Arachidonoylglycerol-based fluorescence assay for DH-463, a fluorescent activity-based probe for monoacylglycerol lipase.
Biochem/physiol Actions
Glycerol kinase catalyzes the MgATP-dependent phosphorylation of glycerol to produce sn-glycerol-3-phosphate and is the rate limiting enzyme in the utilization of glycerol. It is also subject to feedback regulation by fructose-1,6-bisphosphate.Mutations in this gene are associated with Glycerol Kinase Deficiency (GKD), a condition characterized primarily by hypertriglyceridemia and hypoglycemia. This enzyme is useful for enzymatic determination of glycerol and triglyceride when coupled with glycerol-3-phosphate dehydrogenase (=G-3-P DH, G3D-301), glycerol-3-phosphate oxidase (=G-3-P oxidase, G3O-301, G3O-311, G3O-321) or pyruvate kinase (PYK-301) and lactate dehydrogenase (LCD-209, LCD-211), lipoprotein lipase (LPL-311, LPL-314) in clinical analysis
Physical form
Lyophilized powder containing phosphate buffer salts and sodium gluconate
Other Notes
One unit will convert 1.0 μmole of glycerol and ATP to L-α-glycerophosphate and ADP per min at pH 9.8 at 25 °C in a coupled system with PK/LDH.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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