B7401

Anti-Mouse IgG (Fc specific)–Biotin antibody produced in goat

Name: Anti-Mouse IgG (Fc specific)–Biotin antibody produced in goat
Brand: SIGMA

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Goat Anti-Mouse IgG

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.46

MDL number:

MFCD00162627

Key Documents

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Product Name

Anti-Mouse IgG (Fc specific)–Biotin antibody produced in goat, affinity isolated antibody, buffered aqueous solution

biological source

goat

conjugate

biotin conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

direct ELISA: 1:160,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400
western blot (chemiluminescent): 1:300,000 using total cell extract of HeLa cells (5-10 μg/well)

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

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1 of 4

This Item	B9904	B8895	A1682
Sigma-Aldrich B7401 Anti-Mouse IgG (Fc specific)–Biotin antibody produced in goat	Sigma-Aldrich B9904 Anti-Mouse IgG (Fc specific)–Biotin antibody produced in goat	Sigma-Aldrich B8895 Anti-Rabbit IgG (whole molecule)–Biotin antibody produced in goat	Sigma-Aldrich A1682 Anti-Mouse IgG (Fab specific)–Alkaline Phosphatase antibody produced in goat
technique(s) direct ELISA: 1:160,000, western blot (chemiluminescent): 1:300,000 using total cell extract of HeLa cells (5-10 μg/well), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400	technique(s) direct ELISA: 1:50,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:300, western blot: 1:200,000-1:400,000 using total cell extract of HeLa cells	technique(s) direct ELISA: 1:200,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:800, western blot: 1:400,000-1:800,000 using using an assay detecting actin in total cell extract of HeLa cells (5-10 μg per well)	technique(s) direct ELISA: 1:40,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:10, western blot: 1:200,000-1:400.000 using assay detecting β-Actin in total cell extract of HeLa cells (5-10 μg per well)
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody
conjugate biotin conjugate	conjugate biotin conjugate	conjugate biotin conjugate	conjugate alkaline phosphatase conjugate
biological source goat	biological source goat	biological source goat	biological source goat
clone polyclonal	clone polyclonal	clone polyclonal	clone polyclonal
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. 2-8°C

Application

Anti-Mouse IgG (Fc specific)−Biotin antibody produced in goat has been used in ELISA and quantitative suspension array technology (qSAT) multiplex antibody assay.

Goat polyclonal anti-Mouse IgG (Fc specific)–Biotin may be used to detect mouse IgG within serum or other biological systems. The antibody may be used as a secondary antibody in immunochemical assays based on biotin:avidin second antibody signal systems wherein mouse IgG is the primary antibody.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Goat polyclonal anti-Mouse IgG (Fc specific)–Biotin reacts with mouse IgG and mouse IgG, Fc fragment. It shows no reactivity with mouse IgG Fab fragment, human IgG, IgA, IgM, kappa and lambda light chain, bovine IgG and IgM, or horse IgG.

Mouse IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in mouse serum. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.
Biotin:avidin second antibody signal systems involve the conjugation of biotin to a secondary antibody. Once the secondary antibody binds to a target primary antibody in an immunochemical assay, the signal is developed by allowing avidin which has been conjugated to an enzyme or signal molecule to bind to the antibody associated biotin molecule.

Other Notes

Antibody adsorbed with bovine, equine and human serum proteins.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

Adsorbed to reduce background staining with bovine, horse or human samples.

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Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number

0000426487

0000407506

0000301487

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