The concentration of this product varies from lot to lot and may range from 2.0 - 5.0 mg/mL. This lot-specific concentration if reported in the Certificate of Analysis. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/b8895#product-documentation
B8895
Anti-Rabbit IgG (whole molecule)–Biotin antibody produced in goat
affinity isolated antibody, buffered aqueous solution
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About This Item
Conjugate:
biotin conjugate
Clone:
polyclonal
Application:
ELISA (d), IHC (p), WB
Citations:
86
Pricing and availability is not currently available.
biological source
goat
Quality Segment
conjugate
biotin conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
technique(s)
direct ELISA: 1:200,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:800, western blot: 1:400,000-1:800,000 using using an assay detecting actin in total cell extract of HeLa cells (5-10 μg per well)
storage temp.
−20°C
target post-translational modification
unmodified
General description
IgG antibodies regulate several functions such as phagocytosis and complement activation. Thus they play a crucial role in facilitating cytological immune responses. Anti-rabbit IgG (whole molecule)–biotin antibody can be used in immunohistochemistry. The product shows minimal interspecies cross reactivity to human IgG hence can be used in enzyme immunoassays or dot blot in presence of human IgG. Anti-rabbit IgG (whole molecule)–biotin antibody reacts specifically to all rabbit immunoglobulin but has no reactivity with human IgG.
IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins
Anti-Rabbit IgG (whole molecule)-Biotin antibody reacts with rabbit serum and rabbit IgG. The product does not cross-react with human IgG.
Anti-Rabbit IgG (whole molecule)-Biotin antibody reacts with rabbit serum and rabbit IgG. The product does not cross-react with human IgG.
Immunogen
Purified rabbit IgG
Application
Anti-Rabbit IgG (whole molecule)-Biotin antibody has been used in
- Immunofluorescence[1]
- Immunohistochemistry
- Western blot
- Direct enzyme linked immunosorbent assay (ELISA)
Biochem/physiol Actions
Avidin, Streptavidin or ExtrAvidine show strong affinity interaction with biotin rendering the biotin-avidin system an extremely effective tool in molecular biology, protein chemistry and immunology. The high specificity of the biotinylated antibody to rabbit IgG and the stability of the biotin-avidin complex has been exploited in variety of reagents (Avidin, Streptavidin or ExtrAvidin conjugated to FITC, TRITC, Peroxidase or Alkaline Phosphatase), for the detection and quantitation of rabbit IgG.
Binds all rabbit Igs.
Physical form
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide
Preparation Note
Adsorbed to reduce background staining with human samples.
Other Notes
Antibody adsorbed with human IgG.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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This Item | |||
|---|---|---|---|
| biological source goat | biological source goat | biological source mouse | biological source goat |
| antibody form affinity isolated antibody | antibody form affinity isolated antibody | antibody form purified immunoglobulin | antibody form affinity isolated antibody |
| conjugate biotin conjugate | conjugate biotin conjugate | conjugate biotin conjugate | conjugate biotin conjugate |
| technique(s) direct ELISA: 1:200,000, western blot: 1:400,000-1:800,000 using using an assay detecting actin in total cell extract of HeLa cells (5-10 μg per well), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:800 | technique(s) direct ELISA: 1:20,000 | technique(s) direct ELISA: 1:60,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,500, western blot: 1:200,000-1:400,000 using using an assay detecting actin in total cell extract of HeLa cells (5-10 μg per well) | technique(s) direct ELISA: 1:200,000-1:300,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200, western blot: 1:400,000-1:800,000 using total cell extract of HeLa cells |
| form buffered aqueous solution | form buffered aqueous solution | form buffered aqueous solution | form buffered aqueous solution |
| clone polyclonal | clone polyclonal | clone RG-16, monoclonal | clone polyclonal |
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Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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