11579681001
Roche
RNase, DNase-free, High Concentration
from bovine pancreas
Synonym(s):
Rnase
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
41105600
Recommended Products
biological source
bovine pancreas
Quality Level
form
solution
specific activity
≥30 U/mg
packaging
pkg of 1 mg (10 mg/ml)
manufacturer/tradename
Roche
concentration
10 mg/mL
General description
A heterogeneous mixture of ribonucleases specially prepared to be free of deoxyribonuclease activity according to the current Quality Control procedures.
Application
High concentration, DNase-free RNase has been used for:
- Genomic DNA isolation
- Isolation of plasmid DNA
- Degradation of RNA for flow cytometry
Quality
Absence of DNase activity tested according to the current Quality Control procedures.
Unit Definition
One unit is the enzyme activity that causes a decrease in absorbance of A0 to A1 within 1 minute under assay conditions: A0 to A1 corresponds to the total conversion, A1 being the final absorbance.
Preparation Note
Working concentration: 50 μg/ml is the recommended working concentration of RNase, DNase-free, for the isolation of genomic DNA.
Working solution: Recommended in dilution buffer: 10 mM Tris-HCl, 5 mM CaCl2, 50% glycerol (v/v), pH 7.0
Working solution: Recommended in dilution buffer: 10 mM Tris-HCl, 5 mM CaCl2, 50% glycerol (v/v), pH 7.0
Other Notes
For life science research only. Not for use in diagnostic procedures.
Unlike most RNase preparations, no boiling before use is necessary to remove DNase activity.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
BET Bromodomain Inhibition as a Therapeutic Strategy to Target c-Myc
Jake E, et al.
Cell, 146(6) (2011)
Alba Font-Tello et al.
Nature protocols, 15(8), 2503-2518 (2020-06-28)
Fixed-tissue ChIP-seq for H3K27 acetylation (H3K27ac) profiling (FiTAc-seq) is an epigenetic method for profiling active enhancers and promoters in formalin-fixed, paraffin-embedded (FFPE) tissues. We previously developed a modified ChIP-seq protocol (FiT-seq) for chromatin profiling in FFPE. FiT-seq produces high-quality chromatin
Diana Pacheco Sousa et al.
STAR protocols, 3(4), 101916-101916 (2023-01-04)
This protocol describes the synthesis and characterization of gold nanoparticle-based nanobeacons as a theranostic strategy for the recognition, detection, and inhibition of miRNA and mRNA. This system is designed for an in vitro evaluation of a sequence's silencing potential and later
Artyom A Alekseyenko et al.
Current protocols in molecular biology, 109, 21-21 (2015-01-07)
In order to understand how chromatin complexes function in the nucleus, it is important to obtain a comprehensive picture of their protein, DNA, and RNA components, as well as their mutual interactions. This unit presents a chromatin cross-linking approach (BioTAP-XL)
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service