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RNase A

Name: RNase A
Brand: ROCHE

from bovine pancreas

Synonym(s):

Rnase

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About This Item

UNSPSC Code:

12352204

EC Number:

3.1.27.5 (BRENDA, IUBMB)

NACRES:

NA.54

Specific activity:

50 U/mg (+25°C, RNA as substrate)

Biological source:

bovine pancreas

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Properties

biological source

bovine pancreas

form

dry powder

specific activity

50 U/mg (+25°C, RNA as substrate)

mol wt

M_r = 13,700

packaging

pkg of 100 mg (10109169001), pkg of 25 mg (10109142001)

manufacturer/tradename

Roche

parameter

65 °C optimum reaction temp.

technique(s)

flow cytometry: suitable

optimum pH

7.0-7.5

storage temp.

2-8°C

Description

General description

Bovine pancreatic RNase A is a member of the RNase A protein superfamily. It is one of the most characterized proteins, and is a kidney shaped basic protein. This protein is composed of 124 amino acids. In its native form, RNase A exists as a homodimer.

Application

For analytical purposes
Isolation of DNA (for this purpose, RNase A should be boiled)
For cell cycle analysis by flow cytometry and propidium iodide (PI) staining

Biochem/physiol Actions

RNase A catalyzes the transphosphorylation and degradation of RNA. It was the first protein identified to exhibit DNA melting functionality. This enzyme binds to several binding sites on single stranded (ss) RNA polynucleotide chain before degrading it. It interacts with ssDNA in a similar manner, which is responsible for its ‘DNA unwinding′ property. RNase A dimer obtained through tandemization shows toxicity to cancer cells.

Preparation Note

RNase A can be dissolved at a concentration of 1 to 10 mg/ml in 10 mM Tris-HCl, pH 7.5, 15 mM NaCl, heated to 100 °C for 15 minutes to inactivate contaminating DNases and cooled slowly to room temperature and dispend into aliquots. Roche recommends subsequent storage at -15 to -25 °C.

Other Notes

For life science research only. Not for use in diagnostic procedures.

One unit is the enzyme activity that causes a decrease in absorbance of A₀ to A₁ within 1 minute under assay conditions. A₀ to A₁ corresponds to the total conversion, A₁ being the final absorbance.

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This Item	R6513	R5250	R5500
Roche RNASEARO RNase A	Sigma-Aldrich R6513 Ribonuclease A from bovine pancreas	Sigma-Aldrich R5250 Ribonuclease A from bovine pancreas	Sigma-Aldrich R5500 Ribonuclease A from bovine pancreas
description from bovine pancreas	description Molecular Biology, ≥70 Kunitz units/mg protein, lyophilized	description Type X-A, ≥90% (SDS-PAGE), ≥70 Kunitz units/mg protein	description Type XII-A, ≥90% (SDS-PAGE), 75-125 Kunitz units/mg protein
biological source bovine pancreas	biological source -	biological source bovine pancreas	biological source bovine pancreas
specific activity 50 U/mg (+25°C, RNA as substrate)	specific activity ≥70 Kunitz units/mg protein	specific activity ≥70 Kunitz units/mg protein	specific activity 75-125 Kunitz units/mg protein
technique(s) flow cytometry: suitable	technique(s) -	technique(s) -	technique(s) cell based assay: suitable
form dry powder	form lyophilized	form buffered aqueous solution	form lyophilized powder
mol wt M_r = 13,700	mol wt 13.7 kDa, ~13,700	mol wt ~13,700	mol wt ~13,700
storage temp. 2-8°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P304 + P340 - P342 + P311 - P501

Hazard Classifications

Resp. Sens. 1

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