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CRISPR11

Sigma-Aldrich

CRISPR-Lenti Human Positive Control DNA

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About This Item

UNSPSC Code:
41106609
NACRES:
NA.51

form

liquid

Quality Level

200

packaging

vial of 50 μL

concentration

20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)

application(s)

CRISPR

shipped in

dry ice

storage temp.

−20°C

General description

This control is intended for use as a positive control for knockout of human gene targets when used with clones of the Sigma lenti CRISPRs. The DNA format of this control construct permits direct transfection in knockout experiments or use in the viral packaging process (for lentiviral-based knockdown experiments).

Validated CRISPR site, which serves as an experimental control for the Sigma lenti CRISPRs. A one-component positive control system consisting of a EF1-alpha-driven Cas9, a U6-driven guide RNA plasmid targeting the human EMX1 gene, with both Puromycin and GFP co-expressed with Cas9. The Sigma lenti CRISPR Human EMX1 Positive Control Vector is a lentiviral-based plasmid vector that contains a guide RNA sequence targeting the human EMX1 (empty spiracles homeobox 1) gene. EMX1 belongs to the EMX homeobox family. The vector backbone is the same as the Sigma lenti CRISPR products.

Application

Functional Genomics/Screening/Target Validation
  • Positive control for creating gene knockouts/genetic modifications in cell lines.

To see more application data, protocols, vector maps visit sigma.com/crisprs.

Features and Benefits

Serves as an experimental control for the CRISPR editing workflow using lenti CRISPRs. Allows for validation of your system with the lenti CRISPR/Cas9 system. A positive result in a mis-match detection assay will indicate validation of your system.

Physical form

Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells, respectively. Also, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids (Product No. SHP001). The human EMX1 positive Control Vector is provided as 1 ug of plasmid DNA in Tris-EDTA (TE).

Legal Information

CRISPR Use License Agreement

Lentiviral and WPRE License Agreements

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

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Ray A Ohara et al.
Arthritis & rheumatology (Hoboken, N.J.), 71(8), 1241-1251 (2019-03-13)
To explore the intrinsic role of inhibitor of DNA binding 1 (ID-1) in rheumatoid arthritis (RA) fibroblast-like synoviocytes (FLS) and to investigate whether ID-1 is citrullinated and autoantigenic in RA. RA patient serum ID-1 levels were measured before and after

Protocols

CRISPR Cas 9 Nuclease RNA-guided Genome Editing

Learn about CRISPR Cas9, what it is and how it works. CRISPR is a new, affordable genome editing tool enabling access to genome editing for all.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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