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SAB4200053

Sigma-Aldrich

Anti-DNA polymerase δ p125 antibody, Rat monoclonal

clone PDG 5G1, purified from hybridoma cell culture

Synonym(s):

Anti-POLD1, Anti-Polymerase (DNA directed), delta 1, catalytic subunit 125kDa, Monoclonal Anti-DNA polymerase δ p125 antibody produced in rat

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rat

Quality Level

200

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

PDG 5G1, monoclonal

form

buffered aqueous solution

mol wt

antigen ~125 kDa

species reactivity

human, mouse, rat

packaging

antibody small pack of 25 μL

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 0.5-1.0 μg/mL using Jurkat cells extract

UniProt accession no.

P28340

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... POLD1(5424)

Related Categories

General description

Monoclonal Anti-DNA polymerase d p125 (rat IgG2a isotype) is derived from the hybridoma PDG 5G1 produced by the fusion of mouse myeloma cells (P3X63Ag8.653) and splenocytes from rat immunized with human p125. The catalytic subunit p125 (POLD1), which is encoded by the Pold1 gene, is highly conserved among eukaryotes. Pold1 comprises of two primary functional domains: an exonuclease domain near the N-terminus and the subsequent polymerase domain.

Immunogen

human p125

Application

Monoclonal Anti-DNA polymerase δ p125 antibody produced in rat has been used in:
  • immunoblotting
  • immunoprecipitation
  • immunocytochemistry
  • co-immunoprecipitation

Biochem/physiol Actions

The exonuclease domain of the catalytic subunit p125 (POLD1) has the ability to catalyze 3′−5′ exonucleolytic proofreading and the polymerase domain helps to catalyze DNA synthesis. Mutations in this subunit have been implicated in mammalian embryogenesis and tumorigenesis.

Physical form

Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Human DNA polymerase delta is a pentameric holoenzyme with a dimeric p12 subunit
Khandagale P, et al.
Life science alliance, 2(2) (2019)
Eukaryotic DNA polymerases, a growing family
Huubscher U, et al.
Trends in Biochemical Sciences, 25(3), 143-147 (2000)
DNA Polymerase delta Is Required for Early Mammalian Embryogenesis
Uchimura A, et al.
Testing, 4(1) (2009)
Prashant Khandagale et al.
Life science alliance, 2(2) (2019-03-20)
Human DNA polymerase delta (Polδ), a holoenzyme consisting of p125, p50, p68, and p12 subunits, plays an essential role in DNA replication, repair, and recombination. Herein, using multiple physicochemical and cellular approaches, we found that the p12 protein forms a

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