C6-BU-1

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514
• Biological source: rat brain
• Relevant disease(s): cancer
• Growth mode: Adherent
• Karyotype: modal No. of chromosomes is 42
• Morphology: fibroblastic/fusiform
• Manufacturer/tradename: ECACC

Properties

• biological source: rat brain
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 08062563-DNA-5UG, pkg of vial of cells 08062563-1VL
• growth mode: Adherent
• karyotype: modal No. of chromosomes is 42
• morphology: fibroblastic/fusiform
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Application

Used in the generation of cell hybrids.

Biochem/physiol Actions

A 8-bromodeoxybromidine resistant mutant of the rat glioma cell line C6. Found to be tumourigenic in BALB/c nude mice and Wistar rats. Strain rattus norvegicus

Rat glioma x rat glioma hybrid

Preparation Note

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 1mM 8-bromodeoxyuridine. The cells can be grown in the absence of bromodeoxyuridine.

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.