Recommended Products
product name
A15, 93040110
biological source
rat brain
growth mode
Adherent
karyotype
Modal no. 42
morphology
Not specified
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Rat, BDIX, glioma
Cell Line Description
A renamed subculture of the rat gliomal line 1A2(R) - 93040129. A15 is tumourigenic in BDIX rats.
Application
Tumourigenicity studies
Culture Medium
DMEM + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:50 to 1:100 i.e. seeding at 2-4x10,000 cells/cm2 flask using 0.25% trypsin/EDTA; 10-15% CO2; 37°C. Cells can reach up to 4x1,000,000 cells per 25cm2 flask.
Other Notes
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Certificates of Analysis (COA)
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