MABS2282

Anti-SIRPA Antibody, clone MY-1

• Synonym(s): Tyrosine-protein phosphatase non-receptor type substrate 1;SHP substrate 1;SHPS-1;Brain Ig-like molecule with tyrosine-based activation motifs;Bit;CD172 antigen-like family member A;Inhibitory receptor SHPS-1;MyD-1 antigen;Signal-regulatory protein alpha-
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: rat
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: MY-1, monoclonal
• mol wt: calculated mol wt 56.14 kDa; observed mol wt ~95 kDa
• purified by: using protein G
• species reactivity: mouse
• packaging: antibody small pack of 100 μL
• technique(s): flow cytometry: suitable; immunofluorescence: suitable; immunoprecipitation (IP): suitable; inhibition assay: suitable; western blot: suitable
• isotype: IgG2aκ
• epitope sequence: Unknown
• Protein ID accession no.: NP_001171118
• UniProt accession no.: P97797
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: mouse ... Sirpa(19261)

Description

General description

Tyrosine-protein phosphatase non-receptor type substrate 1 (UniProt: P97797; also known as SHP substrate 1, SHPS-1, Brain Ig-like molecule with tyrosine-based activation motifs, Bit, CD172 antigen-like family member A, Inhibitory receptor SHPS-1, MyD-1 antigen, Signal-regulatory protein alpha-1, Sirp-alpha-1, mSIRP-alpha1, p84, CD172a) is encoded by the Sirpa (also known as Myd1, Ptpns1, Shps1, Sirp) gene (Gene ID: 19261) in murine species. SIRPA is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-31), which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 32-373), a transmembrane domain (aa 374-394), and a cytoplasmic domain (aa 395-511). Its extracellular region contains one Ig-like V-type domain (aa 32-137) and two Ig-like C1 type domains (aa 149-248 and 255-343). It is highly expressed in cerebral cortex, brain, spinal cord, cerebellum, and spleen, and at much lower levels in kidney, thymus, heart, lung, and liver. Within the cerebellum, highly expressed throughout the molecular layer, and in synaptic glomeruli in the granule cell layer. SIRPA serves as an immunoglobulin-like cell surface receptor for CD47, and their interactions is reported to mediate negative regulation of several monocyte/ macrophage functions. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. SIRPA also acts as a docking protein and induces translocation of PTPN6, PTPN11, and other binding partners from the cytosol to the plasma membrane. SIRPA is phosphorylated on tyrosine residues in response to stimulation with EGF, growth hormone, insulin, and platelet derived growth factor. It is also reported to mediate negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Four isoforms of SIRPA have been described that are produced by alternative splicing. (Ref.: Sakamoto, M., et al. (2022). Proc. Natl. Acad. Sci. USA. 119(1); e2109923118; Yanagita, T., et al. (2017). JCI Insight. 2(1); e89140).

Specificity

Clone MY-1 is a rat monoclonal antibody that detects Tyrosine-protein phosphatase non-receptor type substrate 1 (SIRPA/CD172a).

Immunogen

Eosinophil-enriched cell fraction from the mouse small intestinal lamina propria.

Application

Quality Control Testing

Evaluated by Western Blotting in Mouse brain tissue extracts.

Western Blotting Analysis: A 1:500 dilution of this antibody detected SIRPA in Mouse brain tissue extracts.

Tested applications

Flow Cytometry Analysis: A representative lot detected SIRPA in Flow Cytometry applications (Verjan Garcia, N., et al. (2011). J Immunol. 187(5):2268-77; Yanagita, T., et al. (2017). JCI Insight. 2(1); e89140).

Immunofluorescence Analysis: A representative lot detected SIRPA in Immunofluorescence applications (Verjan Garcia, N., et al. (2011). J Immunol.;187(5):2268-77; Yanagita, T., et al. (2017). JCI Insight. 2(1):e89140).

Inhibition Assay: A representative lot inhibited the growth of subcutaneously transplanted cancer cells in murine models. (Yanagita, T., et al. (2017). JCI Insight.;2(1):e89140; Sakamoto, M., et al. (2022). Proc Natl Acad Sci USA.;119(1)e2109923118).

Immunoprecipitation Analysis: A representative lot immunoprecipitated SIRPA in Immunoprecipitation applications (Verjan Garcia, N., et al. (2011). J Immunol. 187(5):2268-77; Yanagita, T., et al. (2017). JCI Insight. 2(1):e89140).

Western Blotting Analysis: A representative lot detected SIRPA in Western Blotting applications (Verjan Garcia, N., et al. (2011). J Immunol. 187(5):2268-77; Yanagita, T., et al. (2017). JCI Insight. 2(1):e89140).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-SIRPA, clone MY-1, Cat. No. MABS2282, is a rat monoclonal antibody that detects SIRPA/CD172a and is tested for use in Flow Cytometry, Immunofluorescence, Inhibition Assay, Immunoprecipitation, and Western Blotting.

Physical form

Purified rat monoclonal antibody IgG2a in PBS without preservatives.

Storage and Stability

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable