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PROTMEM

Millipore

ProteoPrep® Membrane Extraction Kit

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

General description

ProteoPrep® Membrane Extraction Kit reagents utilize a powerful detergents for higher loading and resolution of proteins in 2D gels. Protein Extraction Reagent Types 3 and 4 are optimized for proteomic studies. Extraction reagents 3 and 4 are ideal for protein extraction prior to isoelectric focusing and 2D electrophoresis. This kit also includes reagents for the reduction and alkylation of disulfide bonds. The ProteoPrep® Membrane Extraction Kit was designed through a collaboration of Proteome Systems and Sigma research scientists.

Application

ProteoPrep® Membrane Extraction Kit is designed to prepare highly enriched membrane protein solutions from many types of cells. The final protein solution are suitable for 2D gel electrophoresis.

Features and Benefits

  • Innovative detergent preparations - Improved solubility allows for higher protein loads and greater visibility of low abundance proteins in 2D gels.
  • Two pre-mixed solubilization solutions - Removes interfereing non-membrane proteins prior to extractions, resulting in uncluttered 2D arrays.
  • Pre-measured reducing & alkylating reagents - Easy-to-use reagents provide improved IEF resolution.

Legal Information

ProteoPrep is a registered trademark of Merck KGaA, Darmstadt, Germany

Kit Components Also Available Separately

Product No.
Description
SDS

  • T7567Tributylphosphine solution, 200 mM (in N-methyl-2-pyrrolidinone), liquid 5 x 0.5SDS

  • C0356Protein Extraction Reagent Type 4 23 mLSDS

  • A3221Iodoacetamide, Single use vial of 56 mg 5 x 56SDS

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Repr. 1B - Resp. Sens. 1 - Skin Corr. 1A - Skin Sens. 1 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Flash Point(F)

186.8 °F - closed cup

Flash Point(C)

86 °C - closed cup


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Fetuin-A is a known inhibitor of vascular calcification in vitro. In arteries with calcification, there is increased immunostaining for fetuin-A. However, vascular smooth muscle cells (VSMC) do not synthesize fetuin-A, suggesting fetuin-A may be endocytosed to exert its inhibitory effects.

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