TBP is an uncharged reducing agent for disulfide bonds of cysteine residues. This reagent is ideally suited for preparation of protein samples prior to IEF and 2D electrophoresis. Reduction with TBP and subsequent alkylation with iodoacetamide improves resolution, allows greater sample loads, and therefore, improves visualization of low abundance proteins
NSCLC cells with a mesenchymal phenotype have shown a marked reduction in sensitivity to EGFR inhibitors, though the molecular rationale has remained obscure. Here we find that in mesenchymal-like tumor cells both tyrosine phosphorylation of EGFR, ErbB2, and ErbB3 signaling
Journal of chromatography. B, Biomedical sciences and applications, 708(1-2), 285-289 (1998-07-08)
A method was developed for the simultaneous determination of gamma-glutamylglutathione (gamma-GluGSH) and other low-molecular-mass thiol compounds (cysteine, cysteamine, homocysteine, cysteinylglycine, gamma-glutamylcysteine, glutathione and N-acetylcysteine) using high-performance liquid chromatography combined with precolumn fluorescence labeling with ammonium 7-fluorobenzo-2-oxa-1,3-diazole-4-sulphonate (SBD-F). These SBD-labeled thiol
The Journal of organic chemistry, 70(16), 6369-6377 (2005-07-30)
The phosphine-catalyzed [3 + 2]-cycloaddition of 5-methylenehydantoins 4 with the ylides 5, derived from addition of tributylphosphine to the 2-butynoic acid derivatives, 6a-d, gives spiro-heterocyclic products. The camphor sultam derivative 6b gives optically active products. Noteable was that the ylides
Proceedings of the National Academy of Sciences of the United States of America, 112(18), E2317-E2326 (2015-04-23)
The embryonic stem cell (ESC) state is transcriptionally controlled by OCT4, SOX2, and NANOG with cofactors, chromatin regulators, noncoding RNAs, and other effectors of signaling pathways. Uncovering components of these regulatory circuits and their interplay provides the knowledge base to
Protein phosphatase-1 (PP1) is a key regulator of transcription and is targeted to promoter regions via associated proteins. However, the chromatin binding sites of PP1 have never been studied in a systematic and genome-wide manner. Methylation-based DamID profiling in HeLa
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