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PKH26GL

Sigma-Aldrich

PKH26 Red Fluorescent Cell Linker Kit for General Cell Membrane Labeling

Distributed for Phanos Technologies

Synonym(s):

Red PKH membrane dye

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.32
Pricing and availability is not currently available.

packaging

pkg of 1 kit

Quality Level

200

manufacturer/tradename

Distributed for Phanos Technologies

storage condition

protect from light

fluorescence

λex 551 nm; λem 567 nm (PKH26 dye)

detection method

fluorometric

shipped in

ambient

storage temp.

room temp

Related Categories

General description

PKH26 is a red‐fluorescent dye. This lipophilic membrane dye is widely used to determine cell proliferation in heterogeneous cells by flow cytometry.[1]

Application

PKH26 Red Fluorescent Cell Linker Kit is for general cell membrane labeling. It has been characterized in a number of model systems and has been found to be useful for in vitro cell labeling, in vitro proliferation studies and long term, in vivo cell tracking.[2][3] The half-life for elution of PKH26 from labeled rabbit red blood cells is greater than 100 days. This enhanced stability is favorable for long term in vivo studies.
This kit is for general cell membrane labeling. It has been characterized in a number of model systems and has been found to be useful for in vitro cell labeling, in vitro proliferation studies and long term, in vivo cell tracking. The half-life for elution of PKH26 from labeled rabbit red blood cells is greater than 100 days. This enhanced stability is favorable for long term in vivo studies.

Linkage

For additional technical details on PKH and CellVue® Fluorescent Cell Linker Dyes including an extensive bibliography, please visit here.

Legal Information

CellVue is a registered trademark of Phanos Technologies

Kit Components Only

Product No.
Description
  • Diluent C 6 x 10
  • PKH26 Cell Linker in ethanol .5 mL

related product

Product No.
Description
Pricing

Pictograms

FlameExclamation mark
GHS02,GHS07

Signal Word

Danger

Hazard Statements

H225,H319

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

57.2 °F - closed cup

Flash Point(C)

14.0 °C - closed cup

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Certificates of Analysis (COA)

Lot/Batch Number
MKCV7397
MKCV4102
MKCV7789
MKCR6075
MKCQ6477

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Rafi Chapanian et al.
Biomaterials, 33(10), 3047-3057 (2012-01-21)
The in vivo circulation of hyperbranched polyglycerol (HPG) grafted red blood cells (RBCs) was investigated in mice. The number of HPG molecules grafted per RBC was measured using tritium labeled HPGs ((3)H-HPG) of different molecular weights; the values ranged from
Anne Julie Frenette et al.
Journal of neurotrauma, 29(1), 1-18 (2011-08-19)
In the intensive care unit, dopamine agonists (DA) have been used in traumatic brain injury (TBI) patients to augment or accelerate cognitive recovery and rehabilitation. However, the efficacy and safety of DA in this population is not well established. We
Ofra Ben Menachem-Zidon et al.
PloS one, 14(6), e0218081-e0218081 (2019-06-14)
The beneficial effect of mesenchymal stem cells (MSCs) on wound healing is mostly attributed to a trophic effect that promotes angiogenesis. Whether MSCs can contribute to the formation of new blood vessels by direct differentiation is still controversial. Pelvic floor
Tracking antigen-driven responses by flow cytometry: Monitoring proliferation by dye dilution
Paul KW, et al.
Cytometry. Part A : the Journal of the International Society For Analytical Cytology, 73(11), 1019-1034 (2008)
Pablo Reclusa et al.
Scientific reports, 10(1), 6494-6494 (2020-04-18)
In the last decade, extracellular vesicles (EVs) have become a hot topic. The findings on EVs content and effects have made them a major field of interest in cancer research. EVs, are able to be internalized through integrins expressed in
View All Related Papers

Articles

PKH Linker Kits for Fluorescent Cell Labeling

PKH dyes are easy to use and achieve stable, uniform, and reproducible fluorescent labeling of live cells. PKH dyes are non-toxic membrane stains which produce high signal to noise ratio.

PKH Linker Kits for Fluorescent Cell Labeling

PKH dyes are easy to use and achieve stable, uniform, and reproducible fluorescent labeling of live cells. PKH dyes are non-toxic membrane stains which produce high signal to noise ratio.

PKH Linker Kits for Fluorescent Cell Labeling

PKH dyes are easy to use and achieve stable, uniform, and reproducible fluorescent labeling of live cells. PKH dyes are non-toxic membrane stains which produce high signal to noise ratio.

PKH Linker Kits for Fluorescent Cell Labeling

PKH dyes are easy to use and achieve stable, uniform, and reproducible fluorescent labeling of live cells. PKH dyes are non-toxic membrane stains which produce high signal to noise ratio.

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Questions

1–10 of 10 Questions  

  1. If we want to use the PKH to label the membrane of fibroblast, and then co-culture them with tumor cells in order to interact, ¿ Will PKH transfer from fibroblast to tumor cells?

    1 answer

    1. PKH dyes are routinely used in co-culture situations. Because staining is nearly instantaneous, rapid and homogeneous dispersion of cells in dye solution is essential. There should be no excess or unincorporated dye in the medium prior to mixing or co-culturing the cell lines. Free dye will be taken up by all cells. See the links below to the product protocol as well as two additional co-culture studies using PKH dyes:

      Protocol
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/271/475/mini26bul.pdf

      Co-culture in scaffolds
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5155425/

      Co-culture, mixed monolayer, and on Transwell
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5168417/

      Helpful?

  2. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  3. What method of fixation can be used for tissue/cells with the PKH Fluorescent Cell Linker Kit for General Cell Membrane Labeling dyes?

    1 answer

    1. A protocol for visualization of tissue sections can be found in the technical bulletin.  For visualization of stained cells by immunofluorescence or flow cytometry, the cells can be fixed in 2% paraformaldehyde for 15 minutes. The use of other organic solvents will extract the dye from the cells.  If internal labeling is desired, the cells can be permeabilized with saponin (50-75 μg/mL). Here is the link to the Troubleshooting Guide.

      Helpful?

  4. Will Product PKH26GL, PKH26 Red Fluorescent Cell Linker Kit for Phagocytic Cell Labeling Products, stain dead cells?

    1 answer

    1. As long as the cell has an intact membrane, the PKH26 dye can label the cell.

      Helpful?

  5. Does the dye leak from the cells after labeling when using Product PKH67GL, PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling?

    1 answer

    1. In general, slow loss of fluorescence has been observed with PKH1 and PKH2 in in vivo studies and it is likely that PKH67 may also slowly lose fluorescence. Leakage (or loss of staining intensity) may also occur due to cell-to-cell transfer of the dye. The most common cause of cell-to-cell dye transfer is inadequate washing of the cells. Wash cells 3-5 times after labeling and transfer samples to new tubes between washes.

      Helpful?

  6. When using Product PKH67GL, PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling, how long will the cells remain stained after treatment?

    1 answer

    1. Labeled cells that have been washed can be visualized in culture up to 100 days after staining (for non-dividing cells). The dye itself is stable and will divide equally when the cells divide. After staining with PKH dyes, you can observe as many as 8 divisions depending on how brightly the cells were stained initially and the amount of surface area on the cells. Most commonly, 4-6 divisions can be visualized.

      Helpful?

  7. What is the difference between PKH26 Red Fluorescent Cell Linker Kit for Phagocytic Cell Labeling Products PKH26GL and PKH26PCL?

    1 answer

    1. The general labeling kit (Product No. PKH26GL) will label the membrane of the cells.  For phagocytic cells, Product No. PKH26PCL is used.  This kit utilizes dye aggregates for specific labeling of phagocytic cells in the presence of other cell types.

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  8. Product PKH26GL, PKH26 Red Fluorescent Cell Linker Kit for General Cell Membrane Labeling, is a red dye.  Do you offer a kit that stains the cells green?

    1 answer

    1. We offer Product numbers:PKH2GL Both of these kits stain the cells with a dye that emits in the green fluorescent range.

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  9. Are cells lost during the PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling staining process?

    1 answer

    1. Over-labeling of the cells will result in loss of membrane integrity and reduced cell recovery.  Methods for improving cell viability can be found on the troubleshooting guide.

      Helpful?

  10. How many cells can be stained with PKH26 Red Fluorescent Cell Linker Kit for General Cell Membrane Labeling, Products PKH26GL and MINI26?

    1 answer

    1. For Product PKH26GL, when using the concentrations indicated in the product bulletin, you can stain 30 samples (1x107 cells/sample).The kit provides 500 ul of PKH26 dye solution.  You use 4 ul per sample.  There is enough dye in the kit to stain 125 samples.The kit provides 60 mls of Diluent C.  You use 2 mls per sample (1 ml for dilution of the dye, 1 ml for dilution of the cells).  There is enough diluent for 30 samples.You can purchase additional diluent as Product CGLDILIf you need less dye, you can purchase Product MINI26 which contains 100 ul of dye and 10 mls of Diluent C.  This kit contains enough dye to stain 25 samples and enough diluent to stain 5 samples.

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