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Sigma-Aldrich

CellVue® Claret Far Red Fluorescent Cell Linker Mini Kit for General Membrane Labeling

Distributed for Phanos Technologies

Synonym(s):

Far red membrane labeling kit

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.32

packaging

pkg of 1 kit

manufacturer/tradename

Distributed for Phanos Technologies

storage condition

protect from light

technique(s)

flow cytometry: suitable

fluorescence

λex 655 nm; λem 675 nm (CellVue claret dye)

application(s)

cell analysis
detection

detection method

fluorometric

shipped in

ambient

storage temp.

room temp

Related Categories

Application

CellVue Claret kit fluoresces in the far red and may be used for general cell membrane labeling including in vitro cell labeling, in vitro lymphocyte proliferation studies,† in vivo cytotoxicity assays,† and also as a second color for monitoring cell-cell membrane transfer.†
CellVue® Claret Far Red Fluorescent Cell Linker Mini Kit for General Membrane Labeling has been used:
  • to label monocyte-derived macrophages (MDMs) for antibody-dependent cellular phagocytosis assay
  • in labeling of Jurkat cells after the induction of apoptosi
  • in labeling of cells in mice for assaying the retention of tumor cells in lung

Biochem/physiol Actions

The CellVue Claret Fluorescent Cell Linker kit uses a proprietary membrane labeling technology to stably incorporate a far red fluorescent dye with long aliphatic tails (CellVue Claret) into lipid regions of the cell membrane.†

Packaging

This kit provides enough dye for up to 25 samples of 20 million cells each and enough diluent for up to 5 samples of 20 million cells each, when using a 2 mL final staining volume containing a final concentration of 2 x 10-6 M of PKH26 dye. Users must determine the optimal dye and cell concentrations from their cell type(s) and experimental purposes.

Linkage

For additional technical details on Fluorescent Cell Linker Dyes including a comprehensive bibliography, please visit PKH and CellVue®; Claret Fluorescent Cell Linker Dyes.

Legal Information

Distributed for Phanos Technologies, Inc.
Patent Information
CellVue is a registered trademark of Phanos Technologies

Pictograms

FlameExclamation mark
GHS02,GHS07

Signal Word

Danger

Hazard Statements

H225,H319

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2

WGK

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Hori M, et al.
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Yersinia pestis, the bacterial agent of plague, is transmitted by fleas. The bite of an infected flea deposits Y. pestis into the dermis and triggers recruitment of innate immune cells, including phagocytic PMNs. Y. pestis can subvert this PMN response
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International journal of molecular sciences, 22(19) (2021-10-14)
Proper placental development relies on tightly regulated trophoblast differentiation and interaction with maternal cells. Human endogenous retroviruses (HERVs) play an integral role in modulating cell fusion events in the trophoblast cells of the developing placenta. Syncytin-1 (ERVW-1) and its receptor
Jingting Li et al.
Biomaterials, 35(2), 642-653 (2013-10-24)
Autologous cells suffer from limited cell number and senescence during ex vivo expansion for cartilage repair. Here we found that expansion on extracellular matrix (ECM) deposited by fetal synovium-derived stem cells (SDSCs) (FE) was superior to ECM deposited by adult
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Journal of extracellular vesicles, 3, doi:10-doi:10 (2014-07-16)
Tumour-derived extracellular vesicles (EVs) play a role in tumour progression; however, the spectrum of molecular mechanisms regulating EV secretion and cargo selection remain to be fully elucidated. We have reported that cavin-1 expression in prostate cancer PC3 cells reduced the
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