The recommendation to store this product at -20°C is due to the presence of 50% glycerol, which lowers its freezing point to approximately -23°C. Consequently, it should not freeze at -20°C; if it does, it may indicate that the freezer is set too low, not properly calibrated, or that the glycerol ratio is incorrect. If the material is frozen at or below -23°C, or if it contains less or no glycerol, the gel could be damaged, become fragile, and fail to perform as expected.
E6406
EZview™ Red Glutathione Affinity Gel
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About This Item
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analyte chemical class(es)
proteins (GST)
Quality Segment
technique(s)
immunoprecipitation (IP): suitable, protein purification: suitable
storage temp.
2-8°C
General description
EZviewä Red Glutathione Affinity Gel is a highly visible, red colored glutathione agarose affinity gel. The affinity resin is composed of glutathione covalently attached through the sulfur to epoxy-activated 4% cross-linked agarose beads, resulting in a 12-atom spacer.
Application
The product is used for affinity capture, molecular pull-down, or IP of proteins containing glutathione binding sequences, such as glutathione S-transferase, glutathione peroxidase and glyoxalase I.
When performing small-scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview™ Red Affinity Gels greatly reduce the risk of pellet loss. EZview™ resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost.
Features and Benefits
- Increased visibility - Red color reduces risk of incidental aspiration
- Improved recovery of target protein by reduced accidental loss
- Higher reproducibility - More consistent yields
Physical form
1:1 (v/v) suspension in PBS containing 50% glycerol and 15 ppm Kathon
Legal Information
EZview is a trademark of Sigma-Aldrich Co. LLC
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This Item | |||
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| technique(s) immunoprecipitation (IP): suitable, protein purification: suitable | technique(s) affinity chromatography: suitable, immunoprecipitation (IP): suitable | technique(s) affinity chromatography: suitable, immunoprecipitation (IP): suitable | technique(s) western blot: suitable |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| analyte chemical class(es) proteins (GST) | analyte chemical class(es) proteins (Immunoglobulins of various mammalian species) | analyte chemical class(es) proteins (Immunoglobulins of various mammalian species) | analyte chemical class(es) - |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
Storage Class
10 - Combustible liquids
wgk
WGK 3
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Why should E6406 be stored at -20°C, and what could happen if it is stored at a colder temperature?
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