E5529
EZview™ Red Streptavidin Affinity Gel
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About This Item
NACRES:
NA.56
UNSPSC Code:
41106500
Pricing and availability is not currently available.
form
suspension
Quality Segment
shelf life
1 yr
technique(s)
western blot: suitable
matrix
4% agarose
pH
7.2
capacity
~10 μg(biotin per ml of packed gel)
shipped in
wet ice
storage temp.
2-8°C
General description
The EZ View Red Streptavidin Affinity gel is composed of streptavidin, covalently attached to
cyanogen bromide-activated 4% agarose beads. It is designed to capture (pull-down) the biotinylated target molecules, such as proteins, peptides, antibodies, nucleic acids, lectins, receptors and ligands.
cyanogen bromide-activated 4% agarose beads. It is designed to capture (pull-down) the biotinylated target molecules, such as proteins, peptides, antibodies, nucleic acids, lectins, receptors and ligands.
Application
Sutitable for use with immunoprecipitation, western blotting,and enzyme assays.
When performing small-scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview™ Red Affinity Gels greatly reduce the risk of pellet loss. EZview™ resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost.
Features and Benefits
- Increased visibility - Red color reduces risk of incidental aspiration
- Improved recovery of target protein by reduced accidental loss
- Higher reproducibility - More consistent yields
Physical form
1:1 (v/v) suspension in PBS containing 50% glycerol and 15 ppm Kathon
Legal Information
EZview is a trademark of Sigma-Aldrich Co. LLC
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This Item | |||
|---|---|---|---|
| matrix 4% agarose | matrix - | matrix crosslinked agarose | matrix - |
| capacity ~10 μg(biotin per ml of packed gel) | capacity - | capacity ≥0.4 mg/mL, gel binding capacity (HA-tagged fusion protein) | capacity ≥8 mg/mL, gel binding capacity (rabbit IgG) |
| form suspension | form - | form - | form liquid |
| technique(s) western blot: suitable | technique(s) immunoprecipitation (IP): suitable, protein purification: suitable | technique(s) affinity chromatography: suitable, immunoprecipitation (IP): suitable | technique(s) affinity chromatography: suitable, immunoprecipitation (IP): suitable |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
| shipped in wet ice | shipped in - | shipped in - | shipped in - |
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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