Skip to Content
Merck
All Photos(1)

Key Documents

ABT260

Sigma-Aldrich

Anti-Amelogenin, X isoform/AMELX Antibody

serum, from rabbit

Synonym(s):

Amelogenin, X isoform, Leucine-rich amelogenin peptide, LRAP

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

porcine, mouse

technique(s)

immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

mouse ... Amelx(11704)

General description

Amelogenin, X isoform/AMELX, also known as Leucine-rich Amelogenin peptide, or LRAP, and encoded by the gene AMELX/AMG/AMGX, is an important protein in the biomineralization of teeth. AMELX appears to regulate the formation of crystallites during the secretory stage of tooth enamel development. AMELX is a secreted extracellular matrix protein that has many isoforms. Interestingly mutations in the AMELX protein are associated with changes in enamel microstructure that are linked with severe dental disease, and the various isoforms of AMELX protein all aid in increasing enamel thickness of the developing tooth.

Immunogen

Recombinant protein corresponding to mouse Amelogenin, X isoform/AMELX.

Application

Anti-Amelogenin, X isoform/AMELX Antibody is an antibody against Amelogenin for use in western blotting & IHC.
Research Category
Cell Structure
Research Sub Category
ECM Proteins
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Amelogenin, X isoform/AMELX using recombinant mouse Amelogenin179.

Western Blotting Analysis: A representative lot from an independent laboratory detected Amelogenin, X isoform/AMELX in DESC spheres and cervical loop lysates (Chang, J. Y., et al. (2013). J Cell Biol. 288(40):28952-289561.).

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Amelogenin, X isoform/AMELX in DESC spheres and cervical loops (Chang, J. Y., et al. (2013). J Cell Biol. 288(40):28952-289561.).

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Amelogenin, X isoform/AMELX in normal ameloblasts in the wild type and the pseudostratified layer and amorphous matrix of the null mice (Rios, H., et al. (2005). Mol Cell. Biol. 25(24):11131-11144.).

Quality

Evaluated by Western Blotting in pig enamel acid extract.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Amelogenin, X isoform/AMELX in pig enamel acid extract.

Target description

~23 kDa observed. At least 3 isoforms may be observed between 19-23 kDa in pig enamel acid extracts (Chang, J. Y., et al. (2013). J Cell Biol. 288(40):28952-289561.). Uncharacterized band(s) may be observed in some cell lysates.

Physical form

Rabbit polyclonal serum with 0.05% sodium azide.
Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Yikang Ji et al.
Developmental dynamics : an official publication of the American Association of Anatomists, 250(10), 1505-1517 (2021-03-28)
Mutation in Odontogenesis-associated phosphoprotein (ODAPH) has been reported to cause recessive hypomineralized amelogenesis imperfecta (AI) in human. However, the exact role of ODAPH in amelogenesis is still unknown. ODAPH was identified as a novel constituent of the atypical basal lamina
E E Smith et al.
Journal of dental research, 97(10), 1144-1151 (2018-06-08)
Tooth loss is a significant health issue currently affecting millions of people worldwide. Artificial dental implants, the current gold standard tooth replacement therapy, do not exhibit many properties of natural teeth and can be associated with complications leading to implant
Nelson Monteiro et al.
Biomaterials, 106, 167-179 (2016-08-28)
Tissue engineering and regenerative medicine technologies offer promising therapies for both medicine and dentistry. Our long-term goal is to create functional biomimetic tooth buds for eventual tooth replacement in humans. Here, our objective was to create a biomimetic 3D tooth
Elizabeth E Smith et al.
Journal of tissue engineering and regenerative medicine, 11(12), 3326-3336 (2017-01-10)
A long-term goal is to bioengineer, fully functional, living teeth for regenerative medicine and dentistry applications. Biologically based replacement teeth would avoid insufficiencies of the currently used dental implants. Using natural tooth development as a guide, a model was fabricated

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service