T1165
Tris-Tricine-SDS Buffer 10× Concentrate
Synonym(s):
10X TTS Buffer, 10X Tris Tricine SDS running buffer, Tris Tricine SDS transfer buffer
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
41105319
NACRES:
NA.25
Recommended Products
sterility
0.2 μm filtered
Quality Level
form
liquid
pH
8.05-8.30 (25 °C in water, high purity, 1:10)
application(s)
diagnostic assay manufacturing
storage temp.
2-8°C
General description
Tris-Tricine-SDS (TTS) running buffer is the cathode (upper reservoir) buffer for SDS−polyacrylamide gel electrophoresis of proteins using the Schagger and von Jagow method. The Schagger and von Jagow method is designed for the separation of small molecular weight proteins. It differs from the Laemmli method in that the glycine is replaced with tricine and the gel contains 1M Tris-HCl, pH 8.45 instead of 0.375 M Tris-HCl, pH 8.9. Dilution of the 10X TTS buffer produces a 1X cathode buffer containing 100 mM Tris, 100 mM tricine and 0.1% SDS. Recommended running conditions is 125 volts.
Application
Tris-Tricine-SDS Buffer 10× Concentrate is suitable for use as a running buffer for polyacrylamide based separation of bronchoalveolar lavage fluid (BALF).
Other Notes
1M Tris, 1M Tricine, 1% (w/v) SDS, pH approx. 8.2.
Analysis Note
Tested for use as a cathode buffer for Tris-Tricine gel electrophoresis to separate low molecular weight proteins.
related product
Product No.
Description
Pricing
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Nonviral gene delivery to the lung with copolymer-protected and transferrin-modified polyethylenimine
Rudolph C, et al.
Biochim. Biophys. Acta Gen. Subj., 1573(1), 75-83 (2002)
Wataru Ohwada et al.
Biochimica et biophysica acta. Molecular basis of disease, 1866(10), 165851-165851 (2020-06-02)
ERK and Akt have been shown to regulate cell sensitivity to death-inducing stress by phosphorylating GSK-3β, a major modulator of the threshold for mitochondrial permeability transition. Here we examined intra-mitochondrial localization of the pro-survival kinases and their regulation by phosphatases.
Bonnie J Berry et al.
ACS chemical neuroscience, 9(7), 1693-1701 (2018-05-11)
Alzheimer's disease (AD) is characterized by slow, progressive neurodegeneration leading to severe neurological impairment, but current drug development efforts are limited by the lack of robust, human-based disease models. Amyloid-β (Aβ) is known to play an integral role in AD
H Schägger et al.
Analytical biochemistry, 166(2), 368-379 (1987-11-01)
A discontinuous sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) system for the separation of proteins in the range from 1 to 100 kDa is described. Tricine, used as the trailing ion, allows a resolution of small proteins at lower acrylamide concentrations
Rachel E Gerver et al.
Analytical chemistry, 86(21), 10625-10632 (2014-10-01)
We describe a microfluidic Western blot assay (μWestern) using a Tris tricine discontinuous buffer system suitable for analyses of a wide molecular mass range (6.5-116 kDa). The Tris tricine μWestern is completed in an enclosed, straight glass microfluidic channel housing
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service