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Key Documents

L7920

Sigma-Aldrich

β-Lactamase Blend

recombinant, expressed in E. coli

Synonym(s):

Carbapenemase, Cephalosprinase

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About This Item

Enzyme Commission number:
UNSPSC Code:
12352204
NACRES:
NA.54

recombinant

expressed in E. coli

Quality Level

sterility

γ-irradiated

form

lyophilized powder

storage temp.

2-8°C

General description

No detectable growth in tryptone soy broth at 30-35 °C for 14 days

Biochem/physiol Actions

β-Lactamases exhibit high carbapenemase activity. β-lactamases can destroy the β-lactam ring. True carbapenemases has the ability to hydrolyse several β-lactam ring containing carbapenems like meropenem, doripenem, imipenem and ertapenem.

Components

600-1500 IU beta-lactamase I per vial
60-150 IU beta-lactamase II per vial

Unit Definition

One unit will hydrolyze 1.0 μmole substrate per min (βI: benzylpenicillin; βII: cephalosporin C) in phosphate buffer, pH 7.0 at 25°C.

Physical form

Lyophilized powder containing sodium chloride, potassium phosphate, dipotassium phosphate and sorbitol.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Mechanisms and Detection of Antimicrobial Resistance
Principles and Practice of Pediatric Infectious Diseases (Fourth Edition), 1467-1478 (2017)
Identification and screening of carbapenemase-producing Enterobacteriaceae
Nordmann P, et al.
Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases, 18(5), 432-438 (2012)
Lisa M Miller et al.
ACS applied materials & interfaces, 11(36), 32599-32604 (2019-08-27)
Antimicrobial resistance (AMR) has been identified as a major threat to public health worldwide. To ensure appropriate use of existing antibiotics, rapid and reliable tests of AMR are necessary. One of the most common and clinically important forms of bacterial
Matthew D Simmons et al.
Antibiotics (Basel, Switzerland), 9(10) (2020-10-03)
The misuse of antibiotics in health care has led to increasing levels of drug resistant infections (DRI's) occurring in the general population. Most technologies developed for the detection of DRI's typically focus on phenotyping or genotyping bacterial resistance rather than
Jun Hirose et al.
The Journal of experimental medicine, 211(1), 153-163 (2013-12-25)
Signal transducer and activator of transcription 5 (Stat5) is essential for cytokine-regulated processes such as proliferation, differentiation, and survival in hematopoietic cells. To investigate the role of Stat5 in osteoclasts, we generated mice with an osteoclast-specific conditional deletion of Stat5

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