A0418
Anti-Rabbit IgG (whole molecule)–Alkaline Phosphatase antibody produced in goat
IgG fraction of antiserum
Synonym(s):
Goat Anti-Rabbit IgG (whole molecule)–AP
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About This Item
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biological source
goat
conjugate
alkaline phosphatase conjugate
antibody form
IgG fraction of antiserum
antibody product type
secondary antibodies
clone
polyclonal
species reactivity
rabbit
technique(s)
direct ELISA: 1:20,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40
western blot (chemiluminescent): 1:100,000
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
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General description
IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection. Alkaline Phosphatase is an enzyme that catalyzes the conversion of chromogenic substrates such as p-nitrophenylphosphate (PNPP); chemiluminescent substrates such as CDP-Star® and fluorogenic substrates such as 4-methylumbelliferyl phosphate (4-MUP) into detectable chromophores, light-emitters or fluorescers, respectively.
Rabbit IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in rabbit serum. Immunoglobulins (Igs) belongs to the immunoglobulin super-family. Each immunoglobin have two heavy (H) and two light (L) chains, held together by disulphide linkages. Each light chain comprises one variable N-terminal region and a constant C-terminal region. Heavy chain has one variable N-terminal region and three or four constant (CH1-CH4) C-terminal region. The four classes of IgG can be IgG1, IgG2, IgG3, and IgG4.
Specificity
Goat polyclonal anti-rabbit IgG (whole molecule)-alkaline phosphatase antibody reacts with rabbit IgG in vitro, in rabbit serum and biological fluids.
Immunogen
pooled normal rabbit serum
Application
Anti-Rabbit IgG (whole molecule)−Alkaline Phosphatase antibody produced in goat has been used in western blotting and enzyme-linked immunosorbent assay (ELISA).
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
Citrullination of antithrombin by PADI4 was analyzed by ELISA using alkaline phosphatase conjugated goat anti-rabbit IgG as the secondary diluted at 1:5000 in 0.05M carbonate/bicarbonate buffer (Ph 9.6).
Biochem/physiol Actions
IgG1 is most abundant and its deficiency results in hypogammaglobulinemia. IgG2 deficiency increases susceptibility to bacterial infections. IgG3 mediates effector functions and IgG4 is associated with asymptomatic infection. IgG antibody have enormous therapeutic potential and the Fc region contributes for the development of therapeutic antibody.
Physical form
Solution in 0.05 M Tris buffer, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide
Legal Information
CDP-Star is a registered trademark of Tropix, Inc.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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