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Sigma-Aldrich

Goat Anti-Rabbit IgG Antibody, HRP-conjugate

1 mg/mL, Upstate®

Synonym(s):

Anti-rabbit IgG

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.46

biological source

goat

Quality Level

100

conjugate

peroxidase conjugate

antibody form

purified immunoglobulin

antibody product type

secondary antibodies

clone

polyclonal

species reactivity

rabbit

manufacturer/tradename

Upstate®

concentration

1 mg/mL

technique(s)

ELISA: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG

shipped in

dry ice

target post-translational modification

unmodified

Related Categories

General description

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Specificity

Specific for rabbit IgG heavy and light chains.

Immunogen

Highly purified whole rabbit IgG.

Application

Goat Anti-Rabbit IgG Antibody, HRP-conjugate detects level of Rabbit IgG & has been published & validated for use in WB, IH, ELISA.

Quality

Evaluated by western blot.

Western Blot Analysis: Suitable for western blotting (dot blot), ELISA and immunohistochemistry. A working dilution of 1:15,000 to 1:60,000 is suggested for ELISA. A working dilution of 1:2,000 to 1:6,000 is suggested for western blotting procedures. A working dilution of 1:500 to 1:3,000 is suggested for immunohistochemistry. Optimal antibody dilution for other applications should be determined by the researcher.

Physical form

Goat polyclonal conjugated with horseradish peroxidase IgG in buffer containing 50% storage buffer 0.02 M potassium phosphate, 0.15 M NaCl, pH 7.2, 10 mg/mL BSA, 0.01% (w/v) gentamicin sulfate and 50% glycerol.
Rehydration: Add 500 µL sterile, distilled water containing 50% glycerol, to make a 1 mg/mL stock solution.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Methods in molecular biology (Clifton, N.J.), 877, 313-324 (2012-05-23)
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Tubular epithelial cells secrete connective tissue growth factor (CTGF, CCN2), which contributes to tubulointerstitial fibrosis. However, the molecular regulation of CTGF in human primary tubular epithelial cells (hPTECs) is not well defined. Therefore, CTGF expression was characterized in hPTECs isolated
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