AP112P
Goat Anti-Human IgG Antibody, HRP conjugate
Chemicon®, from goat
Synonym(s):
Anti-Human IgG HRP Conjugate
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.46
Recommended Products
biological source
goat
Quality Level
conjugate
peroxidase conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
lyophilized
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
western blot: suitable
isotype
IgG
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
General description
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. It is a glycoprotein and mainly helps in immune defense. About 70 percent of the total immunoglobulin consists of IgG. IgG is a monomeric immunoglobulin, made up of four polypeptide chains. It consists of two 50 kDa heavy chains (gamma) and two 25kDa light chains. These chains are inter-linked by di-sulfide bonds. Each molecule has two antigen binding sites. IgG is the most abundant immunoglobulin. It is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).
Specificity
The antibody reacts with the heavy chains of human IgG and with the light chains common to most human immunoglobulins.
Immunogen
Prepared from purified human IgG.
Application
Goat Anti-Human IgG Antibody, HRP conjugate has been used:
- to detect the bound immunoglobulin G (IgG) in enzyme-linked immunosorbent assay (ELISA) assay
- in immunohistochemistry or cytochemistry at a dilution of 1:500 - 1:5,000
- for western blotting with chromogenic substrates at a dilution of 1:5,000 - 1:100,000 and with ECL substrates at a dilution of 1:10,000 - 1:200,000.
- for EIA at a dilution of 1:5,000-1:100,000
Biochem/physiol Actions
Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. It initiates the classical pathway of the complement system and also neutralizes virus particles and toxins. Maternal IgG can pass through the human placenta to fetus providing immune defense for the neonate against infections. It facilitates antibody-dependent cell-mediated cytotoxicity (ADCC). IgG is digested by papain to produce fragment antigen-binding (Fab). Pepsin digestion of IgG results in the formation of fragment crystallizable (Fc). IgG levels in severe acute respiratory syndrome coronavirus (SARS-CoV)-2 infected patients may be useful to evaluate the severity and prognosis of coronavirus disease 2019 (COVID-19).
Physical form
Purified by immunoaffinity chromatography using antigen coupled to agarose beads. Lyophilized. Buffer is 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA. Contains no preservative.
Storage and Stability
Maintain lyophilized product at 2°-8°C for up to 12 months from date of receipt. After reconstitution the product is stable for six weeks at 2°-8°C as an undiluted liquid. Reconstitute vial with 2 mL of distilled water. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of antibody (and buffer salts) will decrease to one-half of the original after the addition of glycerol and final working dilutions must be adjusted accordingly. Avoid repeated freeze thaw cycles.
WARNING:
Use of sodium azide as a preservative will substantially inhibit the enzyme activity of HRP.
WARNING:
Use of sodium azide as a preservative will substantially inhibit the enzyme activity of HRP.
Other Notes
Research Category: Secondary & Control Antibodies
Research Sub Category
Whole Immunoglobulin Secondary Antibodies
Whole Immunoglobulin Secondary Antibodies
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Sun Woo Lim et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 33(10), 10889-10901 (2019-07-04)
Synthetic biologic drugs are highly successful for induction therapy in transplantation, but the development of novel biologics is limited because of the high cost of synthesis and purification. In this study, we developed a novel strategy for the production of
Fulminant Amoebic Colitis: Role of the Innate and Adaptive Immune Responses
Colitis (2012)
Arinjay Banerjee et al.
Microorganisms, 8(3) (2020-04-05)
The role of bats in the enzootic cycle of Lyme disease and relapsing fever-causing bacteria is a matter of speculation. In Canada, Borrelia burgdorferi sensu stricto (ss) is the genospecies that is responsible for most cases of Lyme disease in
Claudia Tapia-Alveal et al.
Communications biology, 3(1), 781-781 (2020-12-19)
Multiple myeloma (MM) is a neoplasm of plasma cells that secrete patient specific monoclonal immunoglobulins. A recognized problem in MM treatment is the early recognition of minimal residual disease (MRD), the major cause of relapse. Current MRD detection methods (multiparameter
Z Zhong et al.
Clinical and experimental immunology, 195(2), 139-152 (2018-11-14)
Pregnant women and infants are at an increased risk of severe disease after influenza infection. Maternal immunization is a potent tool to protect both these at-risk groups. While the primary aim of maternal influenza vaccination is to protect the mother
Related Content
Products and support for mpox research, offering world-class reagents for immunoassay and molecular assay development.
Products and support for mpox research, offering world-class reagents for immunoassay and molecular assay development.
Products and support for mpox research, offering world-class reagents for immunoassay and molecular assay development.
Products and support for mpox research, offering world-class reagents for immunoassay and molecular assay development.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service