This product is prepared from recombinant human insulin produced in Saccharomyces cerevisiae.
I9278
Insulina solution human
sterile-filtered, BioXtra, suitable for cell culture
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About This Item
origen biológico:
Saccharomyces cerevisiae
Formulario:
solution
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Productos recomendados
origen biológico
Saccharomyces cerevisiae
Nivel de calidad
esterilidad
sterile-filtered
Línea del producto
BioXtra
Formulario
solution
concentración
9.5-11.5 mg/mL
técnicas
cell culture | mammalian: suitable
impurezas
≤50 EU/mL endotoxin
pH
7.9-8.5
Nº de acceso UniProt
temp. de almacenamiento
2-8°C
Clave InChI
PBGKTOXHQIOBKM-FHFVDXKLSA-N
Información sobre el gen
human ... INS(3630)
Descripción general
La insulina es una hormona polipeptídica bicatenaria producida por las células β de los islotes pancreáticos. Su peso molecular es de ~ 5800 Da) Las cadenas α y β se unen por dos enlaces disulfuro intercatenarios. La cadena α contiene un puente disulfuro intracatenario.
Aplicación
La disolución de insulina humana se ha utilizado como suplemento en el medio de Eagle modificado de Dulbecco (DMEM) para cultivar la línea de células epiteliales y la línea de células endometriales humanas, el medio de Eagle esencial mínimo para cultivar la línea de células de adenocarcinoma de mama humanas y el medio 199 para cultivar la línea celular ovárica sana.
La disolución de insulina humana se recomienda para aplicaciones de cultivo celular a 0,5 -1ml por litro de medio.
La disolución de insulina humana se ha utilizado para la prueba de tolerancia a la insulina en ratones y ratas.[1][2] También se ha utilizado para la diferenciación adipogénica de cultivos de células madre embrionarias humanas (hESC) y hMSCs (células del estroma mesenquimatoso humano).[3][4]
La disolución de insulina humana se ha utilizado para la prueba de tolerancia a la insulina en ratones y ratas.[1][2] También se ha utilizado para la diferenciación adipogénica de cultivos de células madre embrionarias humanas (hESC) y hMSCs (células del estroma mesenquimatoso humano).[3][4]
Acciones bioquímicas o fisiológicas
La insulina desempeña un papel en varios procesos celulares, como la promoción del crecimiento celular, la regulación del ciclo celular y la facilitación de la captación de glucosa. Suele utilizarse como suplemento proteico en medios carentes de suero para cultivo de células de mamíferos con el objetivo de contribuir a promover el crecimiento y el mantenimiento óptimos de las células de mamíferos.
Otras notas
InChI: 1S/C257H383N65O77S6/c1-29-131(23)205(313-193(339)104-259)252(393)317-204(130(21)22)248(389)288-159(75-82-200(349)350)217(358)282-156(71-78-189(263)335)221(362)308-183-116-403-404-117-184-243(384)305-178(111-324)240(381)294-162(88-123(7)8)225(366)295-168(95-140-53-61-146(329)62-54-140)228(369)283-154(69-76-187(261)333)218(359)290-161(87-122(5)6)223(364)285-158(74-81-199(347)348)220(361)302-174(101-190(264)336)235(376)298-170(97-142-57-65-148(331)66-58-142)231(372)309-182(242(383)304-176(255(396)397)103-192(266)338)115-402-401-114-181(214(355)273-107-194(340)278-153(72-79-197(343)344)216(357)281-151(51-42-84-271-257(267)268)212(353)272-108-195(341)279-166(93-138-46-36-32-37-47-138)227(368)297-167(94-139-48-38-33-39-49-139)230(371)299-171(98-143-59-67-149(332)68-60-143)238(379)320-208(135(27)327)254(395)322-85-43-52-186(322)246(387)286-152(50-40-41-83-258)222(363)321-209(136(28)328)256(398)399)311-250(391)203(129(19)20)316-236(377)164(90-125(11)12)292-229(370)169(96-141-55-63-147(330)64-56-141)296-224(365)160(86-121(3)4)289-210(351)133(25)277-215(356)157(73-80-198(345)346)287-247(388)202(128(17)18)315-237(378)165(91-126(13)14)293-233(374)173(100-145-106-270-120-276-145)301-239(380)177(110-323)280-196(342)109-274-213(354)180(113-400-405-118-185(310-244(183)385)245(386)319-207(134(26)326)253(394)306-179(112-325)241(382)318-206(132(24)30-2)251(392)312-184)307-226(367)163(89-124(9)10)291-232(373)172(99-144-105-269-119-275-144)300-219(360)155(70-77-188(262)334)284-234(375)175(102-191(265)337)303-249(390)201(127(15)16)314-211(352)150(260)92-137-44-34-31-35-45-137/h31-39,44-49,53-68,105-106,119-136,150-186,201-209,323-332H,29-30,40-43,50-52,69-104,107-118,258-260H2,1-28H3,(H2,261,333)(H2,262,334)(H2,263,335)(H2,264,336)(H2,265,337)(H2,266,338)(H,269,275)(H,270,276)(H,272,353)(H,273,355)(H,274,354)(H,277,356)(H,278,340)(H,279,341)(H,280,342)(H,281,357)(H,282,358)(H,283,369)(H,284,375)(H,285,364)(H,286,387)(H,287,388)(H,288,389)(H,289,351)(H,290,359)(H,291,373)(H,292,370)(H,293,374)(H,294,381)(H,295,366)(H,296,365)(H,297,368)(H,298,376)(H,299,371)(H,300,360)(H,301,380)(H,302,361)(H,303,390)(H,304,383)(H,305,384)(H,306,394)(H,307,367)(H,308,362)(H,309,372)(H,310,385)(H,311,391)(H,312,392)(H,313,339)(H,314,352)(H,315,378)(H,316,377)(H,317,393)(H,318,382)(H,319,386)(H,320,379)(H,321,363)(H,343,344)(H,345,346)(H,347,348)(H,349,350)(H,396,397)(H,398,399)(H4,267,268,271)/t131-,132-,133-,134+,135+,136+,150-,151-,152-,153-,154-,155-,156-,157-,158-,159-,160-,161-,162-,163-,164-,165-,166-,167-,168-,169-,170-,171-,172-,173-,174-,175-,176-,177-,178-,179-,180-,181-,182-,183-,184-,185-,186-,201-,202-,203-,204-,205-,206-,207-,208-,209-/m0/s1
Forma física
estable a 2-8 °C.
Producto relacionado
Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 2
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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Certificados de análisis (COA)
Lot/Batch Number
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Protocolos
Discover our collection of primary human hepatic Kupffer cells and protocol for thawing, plating, and growing Kupffer cells. Find the formulation for Kupffer culture maintenance media.
Discover our collection of primary human hepatic Kupffer cells and protocol for thawing, plating, and growing Kupffer cells. Find the formulation for Kupffer culture maintenance media.
Discover our collection of primary human hepatic Kupffer cells and protocol for thawing, plating, and growing Kupffer cells. Find the formulation for Kupffer culture maintenance media.
Discover our collection of primary human hepatic Kupffer cells and protocol for thawing, plating, and growing Kupffer cells. Find the formulation for Kupffer culture maintenance media.
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How is this insulin produced? In E.Coli? Yeast?...?
1 answer-
Helpful?
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Is this product suitable for insulin tolerance tests in mice
1 answer-
This item has not been specifically tested for suitability in insulin tolerance. However, the product has been used successfully in a number of published papers. Please see the link below to review one such article:
https://pmc.ncbi.nlm.nih.gov/articles/PMC2785638/Helpful?
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How long does the insulin remain potent for if stored in the fridge?
1 answer-
This product is assigned an expiration date of approximately 2 years from the date of Quality Release, when stored as directed at 2-8°C. The expiration date is lot specific and reported on the product label, as well as the Certificate of Analysis. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/i9278#product-documentationHelpful?
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Is this insulin considered of fast or slow action?
1 answer-
This product is intended for research use only and is not a Therapeutic insulin. The research grade insulin products are not defined by the Therapeutic types. There are analytical standards available for each type. Please see the links below to review additional product options for research use:
Insulin Glargine - Slow acting Insulin
https://www.sigmaaldrich.com/search/insulin-glargine?focus=products&page=1&perpage=30&sort=relevance&term=insulin%20glargine&type=productAspart Insulin - Fast acting Insulin
https://www.sigmaaldrich.com/search/insulin-aspart?focus=products&page=1&perpage=30&sort=relevance&term=insulin%20aspart&type=productLispro Insulin - Fast acting Insulin
https://www.sigmaaldrich.com/search/insulin-lispro?focus=products&page=1&perpage=30&sort=relevance&term=insulin%20lispro&type=productHelpful?
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Is this human insulin suitable for use in mouse cell culture?
1 answer-
Yes, this product is suitable for mouse cell culture and has been cell culture tested on the L929 and 3T3 mouse cell lines. Please see the link below to review peer-reviewed articles that may be of interest:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6892261/#:~:text=This%20process%20requires%20DRAM%2D1,and%20permitting%20efficient%20mTORC1%20activation.Helpful?
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what is the morality of the product I9278, Insulin solution human?
1 answer-
The molarity of a 10 mg/mL solution of human insulin, with a molecular weight of 5807 daltons, is approximately 1.7 μmol/mL or 1.7 mM.
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What kind of solution is the insulin (Product number: I9278) dissolved in?
1 answer-
The insulin (cat# I9278) is dissolved in HEPES solution.
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Are there any contaminants (IgG, IgM example) in I9278?
1 answer-
Unfortunately, this product is not tested for impurities.
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What is the content of the insulin solution/buffer (any perservatives and non insulin components?)
1 answer-
This product is prepared in a HEPES buffer, pH 7.9 - 8.5.
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Can you store this product aliquoted at -20 degrees to extend shelf-life?
1 answer-
For research applications, freezing insulin solution has been a common pratice. However, solutions available on the commercial market for therapeutic use are never frozen, in order to maintain maximum activity. There have been no stability studies perform on the shelf life of frozen insulin solutions. Please see the link below to review a publication that may be helpful:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8721649/Helpful?
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