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Documentos clave

I6634

Sigma-Aldrich

Insulina from bovine pancreas

powder, BioReagent, suitable for cell culture

Sinónimos:

Bovine insulin, Cell culture grade insulin

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About This Item

Fórmula empírica (notación de Hill):
C254H377N65O75S6
Número de CAS:
11070-73-8
Peso molecular:
5733.49
Número CE:
234-291-2
Número MDL:
MFCD00131380
Código UNSPSC:
12352209
NACRES:
NA.77
En este momento no podemos mostrarle ni los precios ni la disponibilidad

origen biológico

bovine

Línea del producto

BioReagent

Formulario

powder

potencia

≥25 USP units per mg

técnicas

cell culture | mammalian: suitable
single cell analysis: suitable

solubilidad

acetic acid: 2 mg/mL (pH: 2-3)
hydrochloric acid: 2 mg/mL (pH: 2-3)

Nº de acceso UniProt

P01317

Condiciones de envío

ambient

temp. de almacenamiento

−20°C

Clave InChI

IXIBAKNTJSCKJM-BUBXBXGNSA-N

Información sobre el gen

cow ... INS(280829)

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Aplicación

La insulina se utiliza como factor de crecimiento en muchos sistemas de cultivo de células de mamífero.
  • La insulina del páncreas bovino se ha utilizado para diferenciar las MSC (células madre mesenquimatosas) de los adipocitos.[1]
  • Se ha utilizado para el cultivo de varias células.[2][3][4]
  • También se ha utilizado para estudiar la inhibición del gen de la HMGCS2 (3-hidroxi-3-metilglutaril-CoA (HMG-CoA) sintasa) mediada por insulina.[5]

Acciones bioquímicas o fisiológicas

Hormona polipeptídica bicatenaria producida por las células β de los islotes pancreáticos. Su peso molecular es de ~ 5800 Da) Las cadenas α y β se unen por dos enlaces disulfuro intercatenarios. La cadena α contiene un puente disulfuro intracatenario. La insulina regula la captación, utilización y almacenamiento celulares de glucosa, aminoácidos y ácidos grasos, e inhibe la hidrólisis de glucógeno, proteínas y grasas.

Otras notas

InChI: 1S/C254H377N65O75S6/c1-29-131(24)205(312-192(334)103-256)250(389)317-204(130(22)23)246(385)287-158(74-81-199(344)345)216(355)281-155(70-77-188(260)330)220(359)307-183-116-399-400-117-184-242(381)305-177(110-321)238(377)293-161(87-122(6)7)223(362)294-167(94-139-52-60-145(324)61-53-139)226(365)282-153(68-75-186(258)328)217(356)289-160(86-121(4)5)221(360)284-157(73-80-198(342)343)219(358)301-173(100-189(261)331)233(372)297-169(96-141-56-64-147(326)65-57-141)229(368)308-182(241(380)303-175(253(393)394)102-191(263)333)115-398-396-113-180(212(351)270-106-193(335)277-152(71-78-196(338)339)215(354)280-150(50-41-83-268-254(264)265)210(349)269-107-194(336)278-165(92-137-45-35-31-36-46-137)225(364)296-166(93-138-47-37-32-38-48-138)228(367)298-170(97-142-58-66-148(327)67-59-142)236(375)318-206(135(28)323)251(390)319-84-42-51-185(319)244(383)285-151(49-39-40-82-255)213(352)276-134(27)252(391)392)310-248(387)202(128(18)19)315-234(373)163(89-124(10)11)291-227(366)168(95-140-54-62-146(325)63-55-140)295-222(361)159(85-120(2)3)288-207(346)132(25)274-214(353)156(72-79-197(340)341)286-245(384)201(127(16)17)314-235(374)164(90-125(12)13)292-231(370)172(99-144-105-267-119-273-144)300-237(376)176(109-320)279-195(337)108-271-211(350)179(112-395-397-114-181(309-243(183)382)240(379)275-133(26)208(347)304-178(111-322)239(378)316-203(129(20)21)249(388)311-184)306-224(363)162(88-123(8)9)290-230(369)171(98-143-104-266-118-272-143)299-218(357)154(69-76-187(259)329)283-232(371)174(101-190(262)332)302-247(386)200(126(14)15)313-209(348)149(257)91-136-43-33-30-34-44-136/h30-38,43-48,52-67,104-105,118-135,149-185,200-206,320-327H,29,39-42,49-51,68-103,106-117,255-257H2,1-28H3,(H2,258,328)(H2,259,329)(H2,260,330)(H2,261,331)(H2,262,332)(H2,263,333)(H,266,272)(H,267,273)(H,269,349)(H,270,351)(H,271,350)(H,274,353)(H,275,379)(H,276,352)(H,277,335)(H,278,336)(H,279,337)(H,280,354)(H,281,355)(H,282,365)(H,283,371)(H,284,360)(H,285,383)(H,286,384)(H,287,385)(H,288,346)(H,289,356)(H,290,369)(H,291,366)(H,292,370)(H,293,377)(H,294,362)(H,295,361)(H,296,364)(H,297,372)(H,298,367)(H,299,357)(H,300,376)(H,301,358)(H,302,386)(H,303,380)(H,304,347)(H,305,381)(H,306,363)(H,307,359)(H,308,368)(H,309,382)(H,310,387)(H,311,388)(H,312,334)(H,313,348)(H,314,374)(H,315,373)(H,316,378)(H,317,389)(H,318,375)(H,338,339)(H,340,341)(H,342,343)(H,344,345)(H,391,392)(H,393,394)(H4,264,265,268)/t131-,132-,133-,134-,135+,149-,150-,151-,152-,153-,154-,155-,156-,157-,158-,159-,160-,161-,162-,163-,164-,165-,166-,167-,168-,169-,170-,171-,172-,173-,174-,175-,176-,177-,178-,179-,180-,181-,182-,183-,184-,185-,200-,201-,202-,203-,204-,205-,206-/m0/s1

Forma física

polvo--0 °C; disolución estable a 2 - 8 °C, 1 año

Reconstitución

La insulina tiene baja solubilidad a pH neutro. Puede solubilizarse a 2 mg/ml en ácido acético diluido o ácido clorhídrico, pH 2-3.

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Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000411957
0000411957
0000328086
0000328087
0000324252

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Boah Lee et al.
PloS one, 12(8), e0183569-e0183569 (2017-08-29)
Pancreatic islets can adapt to oscillatory glucose to produce synchronous insulin pulses. Can islets adapt to other oscillatory stimuli, specifically insulin? To answer this question, we stimulated islets with pulses of exogenous insulin and measured their Ca2+ oscillations. We observed
Lei Zhang et al.
Oncotarget, 7(49), 80382-80390 (2016-11-03)
Pleiotrophin (PTN) augments tumor growth by increasing proliferation of tumor cells and promoting vascular abnormalization, but its role in early gliomagenesis has not been evaluated. Through analysis of publically available datasets, we demonstrate that increased PTN mRNA expression is associated
Noncanonical K27-linked polyubiquitination of TIEG1 regulates Foxp3 expression and tumor growth.
Peng DJ et al.
Journal of Immunology, 186, 5638-5638 (2011)
Down-regulation of the mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase gene by insulin: the role of the forkhead transcription factor FKHRL1.
Nadal A et al.
The Biochemical Journal, 366, 289-289 (2002)
Major histocompatibility complex (MHC) class I gene expression in single neurons of the central nervous system: differential regulation by interferon (IFN)-gamma and tumor necrosis factor (TNF)-alpha.
Neumann H et al.
The Journal of Experimental Medicine, 185, 305-305 (1997)
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Questions

1–9 of 9 Questions  

  1. How is your cell culture grade insulin from bovine pancreas product is extracted and purified? Is the pancreas tissue homogenized prior to insulin protein extraction? Are any steps taken to remove contaminants? What is the estimated purity?

    1 answer

    1. The manufacturing method is considered confidential. This product is not tested the purity. The potency is tested for each lot and reported on the Certificate of Analysis.

      Please see the link below to access a sample or lot specific Certificate:
      https://www.sigmaaldrich.com/product/sigma/i6634#product-documentation

      Helpful?

  2. To make a dilution of the stock prepared at 10 mg/ml in HEPES (i-e.10uM which is 34-fold than 2mg/ml) should I still use acidified water, or can i just dilute in PBS or water? This 10 uM dilution will be further dilute 1000 times for cell culture

    1 answer

    1. A stock solution in HEPES at 10 mg/mL, such as that offered as product I0516, may be diluted with water, medium, or the aqueous buffer of choice. This would includes HEPES or PBS. Please see the link below to review the product information sheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/132/262/i6634pis.pdf

      Helpful?

  3. How can i make solution of insulin in HEPES buffer and how this solution is storage?

    1 answer

    1. Preparation of Insulin solution: 10 mg/mL insulin in 25 mM HEPES. Recommended storage temperature 2 - 8 °C.

      Helpful?

  4. How many units of insulin are present in my vial of Product I6634, Insulin from bovine pancreas?

    1 answer

    1. Product No. I6634 has a specification of not less than 27 USP units/mg.  The exact value for the product received can be found the on lot-specific Certificate of Analysis.

      Helpful?

  5. What is the amino acid sequence for Product I6634, Insulin from bovine pancreas?

    1 answer

    1. The sequence and structure of insulin can be found on the product information sheet (under Documents, above).

      Helpful?

  6. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  7. How can I store the insulin solution?  How long?

    1 answer

    1. The USP recommends that solutions of insulin be stored at 2-8°C. It is not recommended to freeze solutions of insulin, as it can aggregate. Sterile solutions should be stable for at least 1 year at 2-8°C.

      Helpful?

  8. What can be used to make a solution of insulin?

    1 answer

    1. Insulin is soluble in dilute acids and alkalis. The solubility of insulin is tested at 2 mg/mL in water (acidified to pH 2 with hydrochloric acid). One can also use 1 mL of water acidified to pH 2 with 10 μL of glacial acetic acid to dissolve 2 mg of insulin. A 10 mg/mL solution of bovine insulin in 25 mM HEPES at pH 8.2 is offered as Product No. I0516.

      Helpful?

  9. What is the comparison between International Units (IU) and USP units for insulin?

    1 answer

    1. The international unit (IU) of insulin is based on the International Standard Preparation of Bioassay (1958). The standard preparation is a mixture of 52% bovine and 48% porcine insulin, containing 24 units/mg. Reference: Martindale: The Extra Pharmacopoeia, 29th ed., p. 392. High purity bovine pancreatic insulin typically assays at approximately 27 USP units per mg. So the IU and USP unit are not identical, but they are approximately 1:1.

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