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Merck
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I6529

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IMDM

With sodium bicarbonate and ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Fabricación farmacéutica

Sinónimos:

Iscove′s MDM, Iscoves MDM, Iscove’s Modified Dulbecco’s Medium, IMDM

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About This Item

MDL number:
MFCD00217469
UNSPSC Code:
12352207
NACRES:
NA.75

product name

Iscove′s Modified Dulbecco′s Medium, With sodium bicarbonate and L-glutamine, liquid, sterile-filtered, suitable for cell culture, suitable for hybridoma

Quality Level

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | hybridoma: suitable
cell culture | mammalian: suitable

impurities

endotoxin, tested

components

glucose: yes
phenol red: yes
NaHCO3: yes
L-glutamine: yes

shipped in

ambient

Storage temp.

2-8°C

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General description

Iscove′s Modified Dulbecco′s Medium contains selenium, additional amino acids and vitamins, sodium pyruvate, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer, and potassium nitrate instead of ferric nitrate, and is a modification of Dulbecco′s Modified Eagle′s medium (DME). Iscove′s medium can be used to support murine B lymphocytes, bone marrow hemopoietic tissue, B cells stimulated with lipopolysaccharide, T lymphocytes, and various hybrid cells.

Application

Iscove′s Modified Dulbecco′s Medium has been used to culture:
  • human hepatocellular carcinoma (HCC) cell line HepG2 as monolayers
  • to culture synovial tissue samples
  • bacterial strain inoculum for stimulation of immune cell experiments

also commonly purchased with this product

Referencia del producto
Descripción
Precios

supplement

Referencia del producto
Descripción
Precios

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Ram C Shankaraiah et al.
Oncogene, 38(45), 7035-7045 (2019-08-15)
Metformin is a hypoglycaemic agent used to treat type 2 diabetes mellitus (DM2) patients, with a broad safety profile. Since previous epidemiological studies had shown that the incidence of hepatocellular carcinoma (HCC) decreased significantly in metformin treated DM2 patients, we
Patricia López et al.
PloS one, 6(9), e24776-e24776 (2011-10-04)
In this work we analyzed the immune activation properties of different Bifidobacterium strains in order to establish their ability as inductors of specific effector (Th) or regulatory (Treg) responses. First, we determined the cytokine pattern induced by 21 Bifidobacterium strains
Ronna Hertzano et al.
The Laryngoscope, 131 Suppl 5, S1-S16 (2020-06-25)
The cellular diversity of the inner ear has presented a technical challenge in obtaining molecular insight into its development and function. The application of technological advancements in cell type-specific expression enable clinicians and researchers to leap forward from classic genetics
Christine Bourgeois et al.
Proceedings of the National Academy of Sciences of the United States of America, 105(25), 8691-8696 (2008-06-20)
A model of chemical thymectomy by inducible Rag ablation was used to study peripheral T cell homeostasis. Induction of Rag ablation was efficient and complete, leading to cessation of thymic T cell production within 3-4 weeks. The decay of peripheral
Gustav Nilsonne et al.
Behavioral and brain functions : BBF, 7, 47-47 (2011-11-22)
Recent advances in cell biology and gene regulation suggest mechanisms whereby associative learning could be performed by single cells. Therefore, we explored a model of classical conditioning in human macrophages in vitro. In macrophage cultures, bacterial lipopolysaccharide (LPS; unconditioned stimulus)
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