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Documentos clave

H9395

Sigma-Aldrich

α-Hemolysin from Staphylococcus aureus

lyophilized powder, Protein ~60 % by Lowry, ≥10,000 units/mg protein

Sinónimos:

α-Toxin

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About This Item

Número de CAS:
94716-94-6
Número MDL:
MFCD00130489
Código UNSPSC:
12352202
NACRES:
NA.56

origen biológico

Staphylococcus aureus

Nivel de calidad

300

Formulario

lyophilized powder

actividad específica

≥10,000 units/mg protein

contiene

sodium citrate buffer as balance

composición

Protein, ~60% Lowry

solubilidad

H2O: soluble 0.49-0.51 mg/mL

Nº de acceso UniProt

Q2G1X0

temp. de almacenamiento

2-8°C

Información sobre el gen

Staphylococcus aureus ... SAOUHSC_01121(3920722)

Descripción general

α-Hemolysin, a pore-forming cytotoxin is an extracellular protein secreted by most strains of pathogenic Staphylococcus aureus. It is secreted as a water-soluble monomer and is a small β-barrel protein.

Aplicación

α-Hemolysin from Staphylococcus aureus has been used:
  • as a component of electrolyte solution for testing pore formation in lipid bilayer using electrophysiological measurements
  • to test its osteogenesis suppressive effects in bone marrow stromal cells (BMSCs)
  • in the preparation of α-hemolysin molecular imprinted polymer (MIP) for Biacore and surface plasmon resonance

α-Hemolysin was used in a study to test the efflux pump and haemolysin activity of Escherichia coli of dairy origin. It was also used to test its adaptation to benzalkonium chloride and the effect of ciprofloxacin on biofilm formation.

Acciones bioquímicas o fisiológicas

α-Hemolysin is selectively hemolytic and the monomeric form binds to a membrane and specific receptors are not required for binding. Upon binding to biological membranes and/or artificial membranes, self-oligomerization occurs, resulting in ring structures (hexameric aggregates) believed to represent transmembrane pores, which are permeable to ions and small metabolites. It has a marked preference for rabbit red blood cells. α-hemolysin stimulates cellular phospholipases and induces a Ca2+ influx. It leads to membrane disruption of the endothelial barrier and leakage of cytoplasmic components and osmotic lysis of the cells. α-hemolysin is implicated in the pathogenesis of sepsis.

Envase

Package size based on protein content

Definición de unidad

One hemolytic unit will cause 50% lysis of a 1% suspension of rabbit red blood cells in phosphate buffered saline, pH 7.0, containing 1% bovine serum albumin after 30 min at 37 °C followed by refrigeration for 30 min at 4 °C.

Pictogramas

Health hazardExclamation mark
GHS08,GHS07

Palabra de señalización

Warning

Frases de peligro

H315,H319,H371

Clasificaciones de peligro

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 2

Órganos de actuación

Lungs,Blood

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000187410
0000187409
0000097039
0000097040
0000187410

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Muhmmad Omar-Hmeadi et al.
Traffic (Copenhagen, Denmark), 19(6), 436-445 (2018-03-16)
Phosphoinositides (PtdIns) play important roles in exocytosis and are thought to regulate secretory granule docking by co-clustering with the SNARE protein syntaxin to form a docking receptor in the plasma membrane. Here we tested this idea by high-resolution total internal
François Vandenesch et al.
Frontiers in cellular and infection microbiology, 2, 12-12 (2012-08-25)
One key aspect of the virulence of Staphylococcus aureus lies in its ability to target the host cell membrane with a large number of membrane-damaging toxins and peptides. In this review, we describe the hemolysins, the bi-component leukocidins (which include
D Fink et al.
Cellular signalling, 1(4), 387-393 (1989-01-01)
Staphylococcal alpha-toxin at subcytotoxic concentrations stimulated phosphatidylinositol turnover and arachidonic acid release in undifferentiated cultures of pheochromocytoma PC12 cells. Stimulation of phospholipase A2 but not C was dependent on extracellular calcium. Addition of staphylococcal alpha-toxin to PC12 cells caused a
Generation of chip based microelectrochemical cell arrays for long-term and high-resolution recording of ionic currents through ion channel proteins
Zheng T, et al.
Sensors and Actuators B, Chemical, 205 (205)
Luning Yu et al.
Biophysical journal, 120(9), 1537-1541 (2021-02-23)
The use of chaotropic reagents is common in biophysical characterization of biomolecules. When the study involves transmembrane protein channels, the stability of the protein channel and supporting bilayer membrane must be considered. In this letter, we show that planar bilayers
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