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Merck

H4777

Sigma-Aldrich

Heparan sulfate proteoglycan

≥400 μg/mL glycosaminoglycan

Sinónimos:

HSPG

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About This Item

Número MDL:
Código UNSPSC:
12352202
NACRES:
NA.75

origen biológico

mouse (Engelbreth-Holm-Swarm mouse sarcoma basement membrane)

Nivel de calidad

descripción

For cell culture use.
Isolated from basement membrane of Engelbreth-Holm-Swarm mouse sarcoma.
Solution in 50 mM Tris HCl, 150 mM NaCl, 1 mM EDTA, 0.1 mM PMSF, pH 7.4, containing ≥400 μg protein per ml.

Formulario

solution

mol peso

≥400 kDa

envase

pkg of 0.1 mg

concentración

≥400 μg/mL glycosaminoglycan

técnicas

cell culture | mammalian: suitable

impurezas

≥100 μg/mL Uronic acid

Nº de acceso NCBI

Nº de acceso UniProt

Especificidad de unión

Peptide Source: Collagen Type IV

Peptide Source: Fibronectin

Peptide Source: Laminin

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

Información sobre el gen

mouse ... Hspg2(15530)

Descripción general

Heparan sulfate proteoglycan (HSPG), a glycoprotein, is an integral part of the basement membrane. It is composed of a core protein covalently bound to heparan sulfate chains. There are three groups of HSPGs: membrane HSPGs, secreted extracellular matrix HSPGs, and the secretory vesicle proteoglycan.

Aplicación

Heparan sulfate proteoglycan has been used:

  • in phosphate buffered saline (PBS) and added to fresh citrated blood samples to achieve trauma-equivalent concentrations in a research study
  • solid phase binding assay
  • for fabrication of extracellular matrix (ECM) microarray slides

Acciones bioquímicas o fisiológicas

Heparan sulfate proteoglycans (HSPGs) have multiple functions in various biological processes. They contribute to basement membrane structure and provide a matrix for cell migration. HSPGs in secretory vesicles, such as serglycin, play a role in packaging granular contents, maintaining proteases activity, and regulating biological activities after secretion. HSPGs can bind and protect cytokines, growth factors, and chemokines, facilitating the formation of gradients essential for cell specification and leukocyte recruitment. They act as receptors for proteases and protease inhibitors, regulate cell-ECM attachment, cell-cell interactions, and cell motility, and can cooperate with growth factor receptors. HSPGs also function as endocytic receptors for ligand clearance and are involved in lipoprotein metabolism and morphogen gradient formation. It also acts as a docking molecule for matrilysin and other matrix metalloproteinases. Heparan Sulfate promotes the attachment of cells in culture and induces high-affinity binding of FGF-basic to cells deficient in heparin sulfate and soluble FGF receptors at a concentration of 10-100 ng/mL.

Componentes

Heparan sulfate proteoglycan is a large biomolecule composed of a core protein covalently bound to heparin sulfate chains that is an integral part of the basement membrane. The number of polysaccharide chains and the size of the core protein are variable according to the source.

Precaución

It is recommended to store this product at 20°C, where it will retain its function for 3 years

Nota de preparación

This product is isolated from the basement membrane of Engelbreth-Holm-Swarm mouse sarcoma in a solution containing 50 mM Tris HCl at pH 7.4, 150 mM NaCl, 1 mM EDTA, and 0.1 mM PMSF.

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)


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C Battaglia et al.
European journal of biochemistry, 208(2), 359-366 (1992-09-01)
A large, low-density form of heparan sulfate proteoglycan was isolated from the Engelbreth-Holm-Swarm (EHS) tumor and demonstrated to bind in immobilized-ligand assays to laminin fragment E3, collagen type IV, fibronectin and nidogen. The first three ligands mainly recognize the heparan
Glycocalyx components affect platelet function, whole blood coagulation, and fibrinolysis: an in vitro study suggesting a link to trauma-induced coagulopathy
Martin W Britten, et al.
BMC anesthesiology, 21(1), 83-83 (2021)
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Journal of molecular biology, 397(3), 826-834 (2010-01-30)
Plasmodium falciparum malaria remains one of the world's leading causes of human suffering and poverty. Each year, the disease takes 1-3 million lives, mainly in sub-Saharan Africa. The adhesion of infected erythrocytes (IEs) to vascular endothelium or placenta is the
Heparan sulfate proteoglycans
Stephane Sarrazin , William C Lamanna, Jeffrey D Esko
Cold Spring Harbor Perspectives in Biology, 3(7) (2011)
Thomas M Clausen et al.
The Journal of biological chemistry, 287(28), 23332-23345 (2012-05-10)
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Uncover more about glycosaminoglycans and proteoglycans including the structure of glycosaminoglycans (GAGs), the different types of GAGs, and their functions.

Uncover more about glycosaminoglycans and proteoglycans including the structure of glycosaminoglycans (GAGs), the different types of GAGs, and their functions.

Uncover more about glycosaminoglycans and proteoglycans including the structure of glycosaminoglycans (GAGs), the different types of GAGs, and their functions.

Uncover more about glycosaminoglycans and proteoglycans including the structure of glycosaminoglycans (GAGs), the different types of GAGs, and their functions.

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