MRC-5 SV2

84100401, human lung (fetal), Fibroblast

• Sinónimos: MRC-5V2 Cells, MRC5V2 Cells
• UNSPSC Code: 41106514

Propiedades

• product name: MRC-5 SV2, 84100401
• biological source: human lung (fetal)
• growth mode: Adherent
• karyotype: Modal no. = 60-80, hyperdiploid
• morphology: Fibroblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Human foetal lung, SV40 transformed

Cell Line Description

Derived from the parental cell line MRC-5 by transformation with the SV40 virus. The cells do not senesce.

Application

Transformation and virus studies: SV40

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11,14
D16S539: 11
D5S818: 11,12
D7S820: 10
THO1: 8
TPOX: 8
vWA: 15

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 1-2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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