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Merck

C6295

Sigma-Aldrich

Cell Freezing Medium-DMSO Serum free 1x

sterile-filtered, suitable for cell culture

Sinónimos:

cryopreservation medium

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About This Item

Código UNSPSC:
12352207
NACRES:
NA.75
En este momento no podemos mostrarle ni los precios ni la disponibilidad

esterilidad

sterile-filtered

Nivel de calidad

Formulario

liquid

técnicas

cell culture | mammalian: suitable
cryopreservation: suitable

impurezas

endotoxin, tested

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

Descripción general

Cell freezing medium is a combination of cryoprotectant DMSO with fetal bovine serum. However serum free medium replaces serum with methylcellulose and poloxamer 188 and other additives like ascorbic acid, α-tocopherol and proline.[1]

Aplicación

Cell Freezing Medium-DMSO Serum free 1x has been used in the freezing of human neural progenitor cells (hNPCs),[2] olfactory ensheathing cells (OECs)[3] and retinal pigment epithelial (RPE) cells.[4]

Otras notas

Complete ready-to-use medium containing Minimum Essential Medium, dimethyl sulfoxide (8.7%) and methyl cellulose. Prepared according to the formulation of Waymouth[5].

Producto relacionado

Referencia del producto
Descripción
Precios

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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Acute and delayed transplantation of olfactory ensheathing cells promote partial recovery after complete transection of the spinal cord
Lopez-Vales R, et al.
Neurobiology of Disease, 21(1), 57-68 (2006)
Cryopreservation of cells using defined serum-free cryoprotective agents
Volbers JC, et al.
Current Directions in Biomedical Engineering, 2(1), 315-318 (2016)
A novel serum-free method for culturing human prenatal retinal pigment epithelial cells
Gamm DM, et al.
Investigative Ophthalmology & Visual Science, 49(2), 788-799 (2008)
Glial cell line-derived neurotrophic factor-secreting human neural progenitors show long-term survival, maturation into astrocytes, and no tumor formation following transplantation into the spinal cord of immunocompromised rats.
Gowing G, et al.
Neuroreport, 25(6), 367-367 (2014)
Chen-Yang Meng et al.
Oncology reports, 43(4), 1169-1186 (2020-04-24)
Osteosarcoma (OS) is the most common primary malignant tumor of the bone affecting children and adolescents. Chemotherapy is now considered as a standard component of OS treatment, not only for children, but also for adults. However, chemoresistance continues to pose

Artículos

Overview of good cell banking practices for cell line cryopreservation purposes.

Overview of good cell banking practices for cell line cryopreservation purposes.

Overview of good cell banking practices for cell line cryopreservation purposes.

Overview of good cell banking practices for cell line cryopreservation purposes.

Questions

  1. Is it possible to thaw this product, prepare aliquots and store them at -20 degrees for further use? Thank you.

    1 answer
    1. The stability of this material following multiple freeze/thaw cycles indicates that a fine precipitate may be evident. However, the performance of the material was is effected. While it is recommended to use this product within 5 days of the initial thaw, it would be up to the end user to continue use following refreezing. Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/241/461/c6295pis.pdf

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