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B8395

Sigma-Aldrich

Anti-Bovine IgG (whole molecule) antibody produced in rabbit

whole antiserum

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About This Item

Número MDL:
Código UNSPSC:
12352203
NACRES:
NA.46

origen biológico

rabbit

Nivel de calidad

conjugado

unconjugated

forma del anticuerpo

whole antiserum

tipo de anticuerpo

secondary antibodies

clon

polyclonal

contiene

15 mM sodium azide

técnicas

indirect ELISA: 1:150,000
quantitative precipitin assay: 4.0 mg/mL

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

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Especificidad

The antiserum is determined to be immunospecific for bovine IgG.

Aplicación

Anti-Bovine IgG (whole molecule) antibody produced in rabbit has been used as a standard to determine protein content by Bradford method using Bio-Rad protein assay dye reagent. It has also been used in several assays to develop a particle concentration fluorescence immunoassay for the quantitative determination of IgG in bovine milk.
Anti-Bovine IgG (whole molecule) antibody produced in rabbit has been used for detecting IgG antibodies in multichannel monolithic quartz crystal microbalance (MQCM).

Acciones bioquímicas o fisiológicas

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.
Immunoglobulins G (IgGi, IgGa) of bovine milk is capable of protecting the gut mucosa against microorganisms.

Forma física

Rabbit Anti-Bovine IgG is supplied as a liquid containing 0.1% sodium azide (see MSDS) as a preservative

Nota de preparación

treated to remove lipoproteins

Almacenamiento y estabilidad

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

nwg


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Development of a particle concentration fluorescence immunoassay for the quantitative determination of IgG in bovine milk
Jack NL, et al.
Journal of Agricultural and Food Chemistry, 41(4), 682-686 (1993)
Effect of thermal protectants on the stability of bovine milk immunoglobulin G
Chao-Cheng C and Hung-Min C
Journal of Agricultural and Food Chemistry, 46(9), 3570-3576 (1998)
Real-time multianalyte biosensors based on interference-free multichannel monolithic quartz crystal microbalance
Jaruwongrungsee K, et al.
Biosensors And Bioelectronics, 67, 576-581 (2015)
S Hashira et al.
Pediatrics international : official journal of the Japan Pediatric Society, 42(4), 337-342 (2000-09-15)
Maternal immunoglobulin G (IgG), transferred across the placenta to the fetus during intrauterine life, is an important component of the neonatal immunological defence mechanisms against infection. There is controversy with respect to differences in placental transfer of the different IgG

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